CRH样肽对大鼠胚泡着床的影响  

Effect of CRH-like Peptide on Blastocyst Implantation in Rats

作　　者：唐美仪[1] 程丽仁[1] 张崇理[1] 

机构地区：[1]中国科学院动物研究所计划生育生殖生物学国家重点实验室,曲阜师范大学生物系

出　　处：《基础医学与临床》1996年第1期57-61,共5页Basic and Clinical Medicine

摘　　要：本文对ＳＤ大鼠子宫内膜提取物作ＣＲＨ放射免疫分析、用免疫组织化学原位定位技术研究ＣＲＨ在大鼠子宫组织的分布，并采用自体对照的大鼠子宫角注射技术观察了ＣＲＨ抗血清对胚泡着床的影响。结果证实：（１）妊娠和非妊娠大鼠子宫内膜都含有ＣＲＨ样物质，其含量分别为：非妊娠大鼠３．６３±０．３２ｐｇ／ｍｇ湿组织（ｎ＝１０），妊娠第４天大鼠４．５７±０．５２ｐｇ／ｍｇ湿组织（ｎ＝８），妊娠第７天大鼠４．３８±０．４９ｐｇ／ｍｇ湿组织（ｎ＝７）。妊娠大鼠与非妊娠大鼠间无显著差异。（２）ＣＲＨ样免疫活性物质存在于子宫内膜固有层结缔组织的若干细胞和内膜腔面的柱状上皮细胞内。（３）当一侧子宫角注射１０μｌＣＲＨ抗血清，对侧子宫角注射１０μｌ正常兔血清（ＮＲＳ）时（ｎ＝６），可见抗血清侧胚泡着床率略高于ＮＲＳ侧，但两侧相比差异不显著；而当一侧子宫角注射２０μｌＣＲＨ抗血清，对侧子宫角注射２０μｌＮＲＳ时（ｎ＝１３），则抗血清侧胚泡着床率明显高于ＮＲＳ侧（Ｐ＜０．０５）。一侧子宫角注射２０μｌ生理盐水或ＮＲＳ与假手术的对侧子宫角相比，着床率无明显差异。结果表明，ＣＲＨ抗血清子宫角局部给药有提高胚泡着床率的作用，其作用方式可能是ＣＲＨ抗血?xtracts of endometrium of Sprague-Dawley rats were measured by the method of radioim-munoassay for corticotropin- releasing hormone(CRH).The distribution of immunoreactive CRHwas observed bv immunohistochemistry technique and the effects of CRH- antiserum on blasto-cyst implantation were observed by local intrauterine administration.The results showed that:(1)CRH-like peptide exists in the endometrium of both pregnant and nonpregnant rats,(2)Im-munoreactive CRH was loCalized both in some cells of the proper lamina and columnar epitheliumin the endometrium.(3)When left uterine horn was inlected with CRH-antiserum and right uter-ine horn inlected with normal rabbits serum(NRS)on day 4 of pregnancy.in 10 μl group(n= 6).the implantation percentage in antiserum side was slightly higher than that in NRS side. but nosignificant difference appears between the both sides.In 20μlgroup(n=13).however.the im-plantation percentage in antiserum side was significantly higher than that in NRS side(P<0.05).(4)No significant change of implantation percentage was brought by injecting 20 μl ofNRS or normal saline when compared to sham-operation.These results suggested that CRH-likepeptide synthesized locally in the endometrium exerted inhibitory effects on blastocyst implanta-tlon.

关 键 词：胚泡 着床 CRH 激素调节 大鼠 

分 类 号：Q132.4[生物学—普通生物学] R321.3[医药卫生—人体解剖和组织胚胎学]