胡萝卜抗冻蛋白的快速与高效纯化  

Fast and Highly Efficient Purification of Carrot Antifreeze Protein

作　　者：张党权[1] 谭晓风[1] 仲健[2] 王金发[3] 

机构地区：[1]经济林育种与栽培国家林业局重点实验室,中南林学院资源与环境学院,湖南长沙410004 [2]细胞产品国家工程研究中心,天津300457 [3]中山大学生命科学学院教育部基因工程重点实验室,广东广州510275

出　　处：《中南林学院学报》2005年第4期27-30,共4页Journal of Central South Forestry University

基　　金：中南林学院引进人才项目"胡萝卜抗冻蛋白基因的植物转化"的部分内容.

摘　　要：将胡萝卜抗冻蛋白在大肠杆菌中进行表达后,对获得的包涵体进行了初步的洗涤纯化.分别采用HPLC和N ativePAGE法对其进行最终的纯化.结果表明,两种方法都能获得高度纯化的目的蛋白,而且耗时都很少.但N ative PAGE法更为经济,且可以同时进行多个样品的纯化,更适用于一般实验室.The inclusion body of carrot antifreeze protein （DcAFP） harvested from the expression in E. coli was primarily purified by washing, and then was finally purified by HPLC and Native PAGE respectively. The purification result shows that both two methods can obtain target protein with high purity and consume little time. However, the method of Native PAGE should be more economical and can be used to purify many samples synchronously, presenting preferable application to a common laboratory.

关 键 词：胡萝卜 抗冻蛋白 纯化 NATIVE PAGE 

分 类 号：S631.2[农业科学—蔬菜学] Q511[农业科学—园艺学]