淹水胁迫下银杏主要生化指标的变化  

Variation of the Main Biochemistry Index of Ginkgo Under Flooding Stress

作　　者：王义强 [1] 谷文众 [2] 姚水攀 [2] 唐隆平  蒋舜村  Xuncun  

机构地区：[1]中南林学院经济林育种与栽培国家林业局重点实验室,湖南,长沙,410004,中南林学院生命科学与技术学院,湖南,长沙,410004 [2]中南林学院生命科学与技术学院,湖南,长沙,410004 [3]东安舜皇山国家森林公园,湖南,永州,425000

出　　处：《中南林学院学报》2005年第4期78-80,85,共4页Journal of Central South Forestry University

基　　金：国家自然科学基金"银杏EST文库的构建与黄酮基因的克隆"的部分内容(编号:30471417).

摘　　要：以3年生的银杏幼树为实验材料,经过淹水处理后,测定不同时间银杏叶片内主要生化指标——丙二醛(M DA)、游离脯氨酸(PRO)、可溶性糖以及超氧化物歧化酶(SOD)含量的变化.结果表明:淹水后的前9天,银杏叶片丙二醛含量无明显变化,第10天,其M DA含量显著增加,达到18.7μm o l/g,比对照M DA含量(7.2μm o l/g)增加1倍多;淹水后第8天,银杏叶片游离脯氨酸(PRO)含量显著增高,达到101.4μg/g,为对照的2倍多;随着淹水时间的延长,银杏叶片可溶性糖的含量逐渐增加,第9天达到32.9 m g/g,随后维持在较高水平上;在淹水后前几天,SOD的活性增加较快,第5天,SOD活性达到最高值854.6 U/g,第6天开始下降,到第11天时,其活性为78.8 U/g,远远低于对照组.A careful analysis was made of the variation of the main biochemistry index, such as MDA, PRO and dissolved sugars, of 3 year-old Ginkgo under flooding stress. The results show that compared to the contrast, the content of MDA in the leaves of Ginkgo did not vary obviously under flooding stress in 9 days; that being 18, 7μmol/g, it changed at the tenth day compared to the contrast, being 7.2μmol/g; that the content of PRO in the leaves of Ginkgo was obviously increased compared to the contrast at the eighth day after flooding stress; that the content of dissolved sugars in the leaves of Ginkgo was becoming more, reaching 32.9 mg/g at the ninth day; and that the activity of SOD in 5 days under flooding stress increased quickly to 854. 6 U/g, then it dropped at the sixth, to 78, 8 U/g at the eleventh, being much lower than that of the contrast.

关 键 词：植物生理 银杏 淹水胁迫 生化指标 丙二醛 脯氨酸 SOD 可溶性糖 

分 类 号：S792.95[农业科学—林木遗传育种] Q946[农业科学—林学]