C-met基因表达、树突状细胞浸润和DNA倍体类型对胃癌转移的影响  

Effect of C-met Gene Expression,Dendritic Cell Infiltration and DNA Ploid Type on Gastric Carcinoma Metastasis

作　　者：冯艳玲[1] 唐建民 叶亚平[1] 

机构地区：[1]河南新乡医学院病理教研室,新乡453003 [2]大庆石油职工医学院免疫教研室,大庆163001

出　　处：《中国临床医学》2005年第4期611-614,共4页Chinese Journal of Clinical Medicine

基　　金：国家自然科学基金资助课题(基金代码:39170440)

摘　　要：目的:探讨c-met基因表达、树突状细胞浸润和DNA倍体类型对胃癌转移和预后的影响。方法:采用SP免疫组化和流式细胞技术对50例胃癌(GC)、11例非典型增生(DYS)、18例慢性萎缩性胃炎伴肠化生(CAG+IM)、15例慢性浅表性胃炎(CSG)组织中的c-met蛋白、树突状阳性细胞、DNA倍体类型进行检测。结果:GC组c-met阳性表达率显著高于CSG组(P<0.05),并与淋巴结转移和浸润深度有关(P<0.05),其余各组间无显著差异。S-100蛋白表达GC组低于其余各组,但无显著差异(P>0.05),伴淋巴结转移组明显低于无淋巴结转移组(P<0.05)。GC组中随着c-met表达增强而S-100表达降低(P<0.05)。c-met阳性率二倍体组低于异倍体组(P<0.05),S-100阳性率二倍体组高于异倍体组(P<0.05)。DNA倍体类型与胃癌患者的病理分期、分化程度及淋巴结转移有关(P<0.05)。DC高表达组术后3年生存率高于低表达组,而c-met高表达组3年生存率低于低表达组(P<0.05)。结论:c-met、S-100蛋白表达和DNA倍体类型与胃癌的生物学行为密切相关,对胃癌中上述指标联合检测能更客观、更全面地判断病人的转移及预后。Objective： To explore the effect of c-met gene expression,dendritic cell infiltration and DNA ploid type on stomach cancer metastasis. Methods： C-met protein, dendritic cell and DNA ploid type were detected in the tissues of 50 gastric carcinomas（GC）, 11 Dysplasia （DYS）, 18 chronic atrophic gastritis with intestinal metaplasia（CAG ＋ IM） and 15 chronic superficial gastritis（CSG）by the way of Immunohistochemistry and flow cytometryc Results： C-met expression rate in GC group was higher than that of CSG group（P〈0. 05）and was related to lymph node metastasia and infiltration depth （P〈0. 01-0. 05）. There was no significantly difference among other groups（P〉0. 05）. S-100 protein expression in GC group was lower than that of other groups. S-100 protein positive in the group with lymph node metastasis was lower than that of the group no lymph node metastasis （P〈0. 05）. With the increase of c-met expression in GC group, S-100 protein expression appears lower gradually. C-met diploid group was lower than that of heteroploid group（P〈0. 05） .S-100 protein positive rate of diploid group was higher than that of heteroploid group （P〈0. 05）. DNA ploid type was related to pathological stage, differention degree and lymph node metastasia（P〈0. 05） ,3 year survival rate after operation in dendrite cell high expression group was higher than that of dendrite cell lower expression group,but 3 year survival rate after operation in high expression group was lower than that of c-met low expression group （P〈0. 05）. Conclusion：C-met, S-100 protein expression and DNA ploid type are closely related to the biological behavior of gastric carcinoma. Detecting the related markers above could objectively judge the metastasis and prognosis of gastric carcinoma.

关 键 词：胃癌 转移 基因 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]