肝癌细胞对三氧化二砷诱导凋亡的敏感性与细胞内谷胱甘肽含量的关系  

The sensitivity of hepatocarcinoma cells to arsenic trioxide associated with level of glutathione in cancer cells

作　　者：任玮玮[1] 李弘[1] 张洹[2] 

机构地区：[1]暨南大学医学院发热研究室,广东广州510632 [2]暨南大学医学院血液病研究所,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2005年第4期486-490,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学重点基金(021195);广州市科技计划(2001-Z-037-01)资助项目

摘　　要：目的:比较肝癌BEL-7402和SMMC-7721细胞对三氧化二砷(As2O3)诱导凋亡的敏感性差异,探讨细胞内谷胱甘肽(GSH)含量与其作用的关系.方法:不同浓度As2O3作用BEL-7402和SMMC-7721细胞24～96 h,流式细胞仪检测细胞DNA含量,计算细胞凋亡百分率;GSH检测试剂盒检测细胞内GSH含量.结果:0.25 μmol/L As2O3作用24 h,有效地诱导BEL-7402细胞凋亡;对SMMC-7721细胞来说,需要用1.0 μmol/L As2O3作用24 h才能达到相同的抑制效果.1.0 μmol/L As2O3作用72 h时,BEL-7402细胞的凋亡率为(43.8±0.2)%,SMMC-7721细胞的凋亡率为(12.8±0.2)%,检测BEL-7402细胞内GSH为(18.7±1.4) nmol/mg;SMMC-7721细胞内GSH为(50.8±5.2) nmol/mg,两者比较均有显著差异.结论:肝癌BEL-7402和SMMC-7721细胞对As2O3诱导细胞凋亡的敏感性不同,可能与细胞内GSH含量有关.Aim： To compare the susceptive differences of arsenic trioxide （As203） induced apoptosis on BEL- 7402 and SMMC - 7721 cells, and explore the effect of GSH level in cells on them. Methods： The flowcytometry was used to detect the apoptotic percentage and GSH kit to determine the level of GSH while the effect of arsenic trioxide on cells of BEL- 7402 cells and SMMC - 7721 cells reached 24 - 96 hours. Results： Arsenic trioxide is able to induce apoptosis of BEL- 7402 cells at 0.25 μmol/L for 24 hours; however, it needed 1.0μmol/L of As2O3 for 24 hours to produce the similar results for SMMC - 7721 cells. The apoptotic rate of BEL-7402 cells is （43.8 ± 0.2）% at dose of 1.0 μmol/L As2O3 for 72 hours, and the apoptofic rate of SMMC - 7721 is （ 12.8 ± 0.2） % only; at this time, level of GSH in BEL-7402 cells is （18.7 ± 1.4） nmol/mg, and in SMMC- 7721 cells is （50.8 ± 5.2） nmol/mg, which showed the significant difference between them. Conclusion： Tne sensibility difference between BEL - 7402 and SMMC - 7721 hepatocarcinoma cells to As2O3 are related with level of GSH in cells.

关 键 词：BEL-7402细胞 SMMC-7721细胞 三氧化二砷 细胞凋亡 谷胱甘肽 

分 类 号：R734[医药卫生—肿瘤]