CIK细胞体外培养的免疫表型  

The immune phenotype of CIK cells in vitro

作　　者：吴昌平[1] 蒋敬庭[1] 邓海峰[1] 陆明洋[1] 李敏[1] 王赫[1] 吴骏[1] 张红宇[1] 季枚[1] 赵伟庆[1] 

机构地区：[1]苏州大学附属第三医院(常州市第一人民医院)肿瘤生物诊疗中心,常州213003

出　　处：《江苏医药》2005年第9期644-645,共2页Jiangsu Medical Journal

基　　金：江苏省卫生厅重大科研课题(K200403)

摘　　要：目的探讨体外培养过程中细胞因子激活的杀伤细胞(CIK)免疫表型的动态变化。方法通过向外周血单个粒细胞(PBMC)中加入白细胞介素2(IL-2)、γIL-1α、γ干扰素(γ-IFN)与抗CD3McAb,诱导出CIK细胞。经流式细胞分析法对CIK细胞进行动态表型分析。结果动态表型分析显示,在第14~21天时CD3+CD16+56+细胞为(31·6±5·5)%^(35·8±9·7)%,呈高表达的平台期。结论CIK细胞在体外培养14~21d,CIK细胞CD3+CD16+56+双阳性细胞呈高表达,此时临床应用可取得较好疗效。Objective To study the dynamic developments of the immune phenotype of CIK cells in vitro. Methods The CIK cells were induced by adding IL-2,γIL-1α,γ-IFN and CD3 McAb in vitro. The immune phenotype of the CIK cells was analized by flow cytometry. Results The immune phenotype was analyzed. The CD3^＋ CD16＋56^＋ of CIK cells was （31.6±5.5）%- （35.8±9.7）% on the 14th day to 21st day, which showed a flat roof with high expression. Conclusion The CD3^＋ CD16 ＋56^＋ of double positive CIK cells has a high expression cultured in vitro on the 14th day to 21st day. A better curative effect may be obtained when used clinically during this period of time.

关 键 词：细胞因子激活的杀伤细胞 细胞因子 免疫表型 肿瘤 细胞体外培养 CIK细胞 抗CD3McAb 激活的杀伤细胞 流式细胞分析法 表型分析 

分 类 号：R135.12[医药卫生—劳动卫生] R730.51[医药卫生—公共卫生与预防医学]