川陈皮素对非小细胞肺癌A549细胞的抑制作用  

The inhibitory effect of nobiletin on human non-small cell lung cancer cell line A549

作　　者：管晓琳[1] 朱玲[1] 周黎明[1] 

机构地区：[1]四川大学华西基础与法医学院药理教研室,四川成都610041

出　　处：《四川生理科学杂志》2005年第2期54-55,共2页Sichuan Journal of Physiological Sciences

摘　　要：目的:观察川陈皮素(5,6,7,8,3′4′-hexamethoxyflavone)对非小细胞肺癌A549细胞的抑制作用并研究其作用机制.方法:以不同浓度的川陈皮素作用于A549细胞,分别用MTT法、细胞生长曲线研究川陈皮素对A549的增殖抑制作用,Giemsa染色观察细胞形态变化.结果:MTT提示川陈皮素浓度为4mg·L-1～128mg·L-1时,对A549的48、72小时抑制率为15%～85%,48小时IC50为25.7mg·L-1,72小时IC50为16.7mg·L-1;生长曲线提示川陈皮素对A549细胞的抑制作用呈明显时效和量效关系;Giemsa染色后细胞出现明显的凋亡形态学变化.结论:川陈皮素对非小细胞肺癌A549细胞具有增殖抑制作用,其机制可能是诱导细胞凋亡.Objective： To investigate the inhibitory effect of nobiletin （5, 6, 7, 8, 3＇4＇-hexamethoxyflavone） on human non-small cell lung cancer cell line A549 and its mechanism of action. Methods： The inhibitory effect of nobiletin on proliferation of A549 cells was evaluated by MTT analysis and growth curve. Its apotosis-inducing effect was demonstrated by morphological observation under microscope（Giemsa staining）. Results： After treated with nobiletin for 48 and 72 hours, MTT assay showed the dose range from 4mg·L^-1 to 128 mg·L^-1 had the inhibitory ratio range from 15 % to 85 %. IC50 of nobiletin to A549 in 48h and 72h were 25.7mg·L^-1 and 16.7 mg·L^-1 respectively; The dose-effct relationship and time-effect relationship were described in the growth curve; The characteristic morphology typical for apoptosis was observed under microscope. Conclusions： Nobiletin could inhibit the growth of A549 cells in vitro, its mechanism is probably associated with the apoptosis inducement.

关 键 词：川陈皮素 A549细胞 凋亡 增殖抑制作用 非小细胞肺癌 GIEMSA染色 诱导细胞凋亡 细胞形态变化 不同浓度 MTT法 

分 类 号：R734.2[医药卫生—肿瘤] R739.5[医药卫生—临床医学]