线栓法建立大鼠局灶性脑缺血模型方法的改进  

A modified suture method of focal cerebral ischemia model in rats

作　　者：任建勋[1] 刘俊[2] 沈映君[1] 

机构地区：[1]成都中医药大学药学院,成都611730 [2]中国人民解放军总后药检所,北京100071

出　　处：《四川生理科学杂志》2005年第2期60-62,共3页Sichuan Journal of Physiological Sciences

摘　　要：目的:提出一个手术简单,重复性好的大鼠局灶性脑缺血模型的改进方法。方法:SD大鼠45只,随机分为3组(15只/组),除空白对照组外,局灶性脑缺血再灌注组与丁基苯酞治疗组均采用大鼠颈总动脉套管进线和分离翼腭动脉但不结扎的方法复制局灶性脑缺血,观察脑梗死体积,脑组织含水量和神经功能缺陷的改变。结果:局灶性脑缺血再灌注比较假手术对照组,脑梗死体积,脑组织含水量和神经功能评分均有明显的差异。结论:改进线栓法复制的大鼠局灶性脑缺血模型可靠,重复性好。Objects： To provide a modified suture method of focal cerebral ischemia model with simple and good repetition in rats.Method： Fouty-five Spragne-Dawley male rats randomly divide into three groups （15/group）. Except the sham-operated control group, the nylon suture was gently advanced from the common carotid artery to the middle cerebral artery in focal cerebral ischemia reperfusion model group and NBP treatment group. But pterygopal artery was not occluded. Results： Compare with the sham-operated control group, the infarct size of brain tissue, the content of water and the neurologic scores of focal cerebral ischemia reperfusion model group was significantly. Conclusion： The modifies method of focal cerebral ischemia by suture in rats was reliable, stable and repeatable.

关 键 词：局灶性脑缺血 线栓法 动物模型 大鼠局灶性脑缺血 模型方法 局灶性脑缺血再灌注 局灶性脑缺血模型 脑组织含水量 脑梗死体积 神经功能缺陷 

分 类 号：R-332[医药卫生]