卵巢癌患者血清胎盘型碱性磷酸酶含量的测定  

DetectionofPlacentalAlkalinePhosphataseinOvarianCancer

作　　者：王瑞秀[1] 宋立新[1] 丁莉莉[1] 孙贻华[1] 吕耀风 

机构地区：[1]山东滨州医学院生化教研室

出　　处：《中华妇产科杂志》1996年第2期107-109,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的：采用特异性检测方法，定量测定卵巢癌患者血清胎盘型碱性磷酸酶（ＰＬＡＰ），为临床可疑患者提供诊断依据。方法：以ＰＬＡＰ为抗原，制得兔抗ＰＬＡＰ抗体，依据抗体免疫特性建立特异的定量检测ＰＬＡＰ固相直接免疫测定法。采用此方法测定８６例健康对照者及８１例恶性肿瘤患者血清ＰＬＡＰ。结果：对照者血清ＰＬＡＰ活性呈偏态分布，第９５百分位数的上限为０．３８Ｕ／Ｌ，以该值作为正常与升高的分界值，恶性肿瘤患者中１８例血清ＰＬＡＰ升高，阳性率为２２．２％，其中卵巢癌患者阳性率为６１．９％。结论：应用固相直接免疫法检测ＰＬＡＰ，对卵巢癌诊断有一定意义。Objective:Toestablishamethodfordiagnosisofmalignanttumorsbydeterminationoftheplacentalalkalinephosphatase(PLAP)inserum.Methods:AsolidphaseenzymeimmunoasaywasadopatedbyanantibodyagainstPLAP.PLAPinserumof81patientswithmalignanttumorand86healthypeopleascontrolweredetermined.Results:Inthecontrolgroup,thePLAPactivityshoweddeviativedistribution,the95thpercentilevaluesofPLAPincontrolgroupwas3.8U/L.Ifthisvalueservedasnormalcut-ofline,18casesamong81patientswithmalignanttumorswerepositive.Thepo-sitiveratewas22.2%,butthepositiveratesinovariancancerwas61.9%.Conclusions:TheincreasingofserumPLAPmaybehelpfulforthediagnosisofmalignanttumorsespecialyovariancancer.

关 键 词：卵巢肿瘤 碱性磷酸酶 胎盘型 血清 

分 类 号：R737.310.2[医药卫生—肿瘤]