JS/1/97/Go株GPMV NP基因的克隆、序列分析  

Cloning and sequencing of NP gene from the JS/1/97/Go strain of GPMV

作　　者：杨玉菊[1] 王君伟[1] 杨志[1] 何海娟[1] 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030

出　　处：《中国预防兽医学报》2005年第5期340-343,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省"十五"攻关课题(GB01B503-02)

摘　　要：根据GenBank中发表的GPMV SF02株的NP基因的核苷酸序列设计合成一对引物,采用RT_PCR扩增出与预期设计的1470 bp大小相符的片断,将此扩增产物克隆入PMD18_T载体,进行序列测定和分析。结果表明:所扩增的NP基因的核苷酸长为1 470 bp,共编码490个氨基酸。同源性分析表明:JS/1/97/Go与国内的SF02株的NP基因核苷酸同源性为98.6%,氨基酸同源性为99.6%。由此可见,鹅副粘病毒JS/1/97/Go分离株与SF02株的亲缘关系较近,同属于新城疫Ⅶ型基因。而其与LaSota株的核苷酸同源性为85.2%,氨基酸同源性为90.8%。说明该毒株相对于经典的NDV在NP基因上已发生了较大的变异。According to the sequence of NP genome of Goose Paramyxovirus （GPMV） SF02 isolate strain which published on GenBank, We designed and synthesized a pair of primers, A 1 470 bp fragment was amplified from JS/1/97/Go strain by means of RT-PCR and cloned into pMD18-T vector, The positive recombinant clone characterized by enzyme digestion and was sequenced. The sequence analysis showed that NP gene consisted of 1470 nucleotides and encoded 490 amino acids. Sequence comparison between JS/1/97/Go and SF02 strain, The homology of the nucleotide was 98.6 % and the amino acid was 99.6 %, which indicated they probably had a common origin , They were both belong to the genotype Ⅶ of NDV virus. The homology of the nucleotide compared with LaSota was 85.2 %and the amino acid was 90.8 %. The result indicated the mutation on the NP gene had varied largely, compared with classic NDV.

关 键 词：鹅副粘病毒 核蛋白基因 克隆 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学] Q785[农业科学—兽医学]