蕲蛇酶减轻大鼠局灶性脑缺血再灌注损伤的实验研究(Ⅱ)  

Effects of Acutobin on Focal Cerebral Ischemia-reperfusion Injury in Rats(Part Ⅱ)

作　　者：林春[1] 徐隽[1] 黄扬[1] 余涓[1] 王晶[1] 陈崇宏[2] 

机构地区：[1]福建医科大学基础医学院,福州350004 [2]福建医科大学药学院,福州3500041

出　　处：《福建医科大学学报》2005年第3期299-302,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金资助项目(C0010012;C021009)

摘　　要：目的从病理形态学观察蕲蛇酶对大鼠局灶性脑缺血再灌注损伤的保护作用并探讨其机制。方法采用线栓法制备大鼠大脑中动脉栓塞后再灌注模型,光镜及电镜观察缺血3h恢复血流再灌24h后脑组织形态学变化,TTC染色观察脑梗死面积,免疫组织化学方法检测脑组织碱性成纤维细胞生长因子(bFGF)的表达。用药组分别在缺血即刻或再灌即刻静脉给予不同剂量的蕲蛇酶,观察比较模型组和蕲蛇酶所有给药组之间上述指标的变化。结果缺血即刻给蕲蛇酶4U/kg组较再灌即刻给蕲蛇酶同剂量组显著减小梗死灶(P<0.001),减轻脑组织病理改变,并使脑组织中bFGF蛋白表达明显增多。结论蕲蛇酶对脑缺血再灌注损伤的神经保护作用可能与脑组织中bFGF表达增高有关;提示该药早期使用更为有效。Objective To further study the neuroprotective effects of acutobin on focal cerebral ischemia reperfusion injury in rats. Methods Reversible middle cerebral artery occlusion（MCAO） models were produced by intraluminal suture technique, and reperfusion was begun at 3 hours after occlusion and lasted 24 hours. The morphology of brain was observed by microscope and electron microscope. The infarct area of brain was measured by 2, 3,5-triphenyltetrazolium chloride（TTC） staining technique; acutobin was administrat- ed iv at different time respectively. After 24 h reperfusion, the infarct area were measured, the change of brain histology and the expression of basic fibroblast growth factor（bFGF） were examined. Results The 4 U/kg dose of acutobin administrated at the beginning of ischemia decreased the infarct area, alleviates the pathological changes and maintain the structure of micro-vessels, significant increases the expression of bFGF. Conclusion Effect of acutobin on MCAO related to enhancing bFGF expression and suggest the administration of acutobin should be more effective at early period use.

关 键 词：蕲蛇 蛇毒凝血酶 再灌注损伤 神经元 成纤维细胞生长因子 碱性 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]