PARP酶抑制剂诱导转化细胞逆转及外源ras癌基因丢失的研究  

Studies on Reversion of a Transformant Line and Loss of Exogenous ras Oncogene after Treatment with an Inhibitor of PARP Enzyme

作　　者：扬其伟 董元舒[1] 陈雅文[1] 陈德风[1] 

机构地区：[1]南开大学分子生物学研究所,天津300071

出　　处：《遗传》1996年第3期6-8,共3页Hereditas（Beijing)

基　　金：国家自然科学基金

摘　　要：用5mmol／LPARP抑制剂苯甲酸胺处理经c－Ha－T24－ras活化癌基因转染得到的转化细胞系，处理4周后发现转化细胞系的某些细胞生物学特性发生了改变，呈现出正常细胞的某些特性．伴随这一现象，检测到细胞中整合的外源T24－ras基因发生了丢失．A transformant line obtained by transfection of NIH3T3 cells with c-Ha-T24-ras gene was treated with 5mmol / L benzamide, an inhibitor of PARP cnzyme for 4 weeks. Some changes in cellular properties of the transformant line werc obscrved. Concomitant with these changes was the deletion of the exogenous c-Ha-T24-ras which had been integrated into genomic DNA of recipient cells.

关 键 词：转化细胞逆转 RAS癌基因 基因丢失 肿瘤 

分 类 号：R730.21[医药卫生—肿瘤]