不同类型表面活性剂对肌酸激酶活力影响研究  

THE EFFECTS OF VARIOUS SURFACTANTS ON THE ACTIVITY OF CREATINE KINASE

作　　者：李学刚[1] 张光先[1] 张明晓[1] 吴光权[1] 董佳里[1] 

机构地区：[1]西南农业大学中心实验室

出　　处：《西南农业大学学报（自然科学版）》1996年第2期125-128,共4页Journal of Southwest Agricultural University

基　　金：国家自然科学基金

摘　　要：本文系统地研究了阴离子Ｃ＿（１２）Ｈ＿（２５）ＳＯ＿４Ｎａ（Ｃ＿（１２）Ｓ），Ｃ＿（１２）Ｈ＿（２５）（ＯＣ＿２Ｈ＿４）＿７ＳＯ＿４Ｎａ（Ｃ＿（１２）Ｅ＿７Ｓ）、阳离子Ｃ＿（１２）Ｈ＿（２５）Ｎ（ＣＨ＿３）＿３·Ｂｒ（Ｃ＿（１２）ＮＭｅ＿３）和非离子Ｃ＿８Ｈ＿（１７）·Ｃ＿６Ｈ＿４·（ＯＣ＿２Ｈ＿４）＿（１０）ＯＨ（ＴＸ－１００）表面活性剂对肌酸激酶（Ｃ·Ｋ·）活力的影响以及变性后的复性。结果表明，Ｃ＿（１２）Ｅ＿７Ｓ和Ｔｘ－１００对Ｃ·Ｋ·的活力破坏较小；Ｃ·Ｋ·经Ｃ＿（１２）Ｅ＿７Ｓ和ＴＸ－１００变性后，冲稀时即可完全复性。Ｃ＿（１２）Ｅ＿７Ｓ不仅对Ｃ·Ｋ·的变性能力强，ｌ０ｍＭ就可使Ｃ·Ｋ·完全失活，而且在高温和常温下能阻止Ｃ·Ｋ·聚集沉淀。Ｃ＿（１２）Ｅ＿７Ｓ是Ｃ·Ｋ·的高效可逆变性剂。The effects of amionic(C12H25SO4Na,C12S;C12H25(OC2H4)7SO4Na;C12E7S)、 Cathmc(C12H25NC(CH3)3·Br,C12NMe3)and non-nionic(C8H17·C6H4·(OC2H4)10OH,TX-100)surfactants on the activity of creatine kinase(C·K·)and the renaturation ofdenaturated protein have been studied.The results show that the effects of CizE75and TX-100 on the activity of C·K·are smaller than that of CizS and C12NMe3.The denaturated C·K·,denaturated by C12E75and TX-100,can be renaturaed when the solutionis diluted.C12E7S is not only strongdenaturant(10 mM is sufficient to lose the activity ofC·K·),but also can prevent the precipitation of C·K·from solution,C12E7Sis thehigh efficiency and reversible denaturant.

关 键 词：肌激酶 表面活性剂 活度 变性 

分 类 号：O647.2[理学—物理化学]