联合转染p^(21)基因及反义c-fos核酸对移植静脉血管平滑肌细胞增殖的影响  

EFFECTS OF GENE p^(21) TRANSFECTION AND ANTISENSE c-fos NUCLEOTIDE ON PROLIFERATION OF VASCULAR SMOOTH MUSCLE CELLS

作　　者：申方[1] 崔瑞耀[2] 叶俊丽[2] 

机构地区：[1]青岛大学医学院附属医院急诊内科,山东青岛2660031 [2]青岛大学医学院病理生理学教研室,山东青岛2660031

出　　处：《青岛大学医学院学报》2005年第3期246-247,共2页Acta Academiae Medicinae Qingdao Universitatis

摘　　要：①目的观察联合转染p21基因及反义c-fos核酸对自体静脉移植后移植血管平滑肌细胞(VSMC)增殖的影响,探讨预防移植血管再狭窄的基因疗法。②方法选择20只新西兰家兔,随机分为实验组和对照组,每组各10只。用显微外科技术建立自体颈静脉-颈总动脉移植模型;实验组移植静脉进行p21基因和反义c-fos核酸转染,而对照组未进行转染。术后2周取出移植血管,用电子显微镜及计算机图像分析仪观察血管中段内膜厚度、管腔狭窄度、平滑肌细胞增殖指数,用免疫组织化学方法对移植血管c-fos、c-myc、移植血管VSMC的增殖细胞核抗原(PCNA)阳性表达进行检测。③结果实验组血管中段内膜厚度、管腔狭窄度、平滑肌细胞增殖指数及c-fos、c-myc、PCNA阳性表达率均明显低于对照组(t=8.19~25.19,t′=6.14、7.36,P<0.01)。④结论移植静脉联合转染P21基因及c-fos反义核苷酸可有效地抑制移植血管VSMC增殖。Objective To observe the effect of transfection of gene p^21 and antlsense c-fos nucleotide on the proliferation of vascular smooth muscle cells （VSMC） after autogenous vein grafts and to investigate the genetic prevention of restenosis in vein grafts. Methods Twenty New Zealand rabbits were divided into two groups： experimental group and control group. Models of autogenous vein carotid artery were set up by using microscopic surgery. Transfection of gene p^21 and antisense c-fos nucleotide was done only in the vein graft of experimental group. The transplanted vascular samples were taken at two weeks after the operation. The intimal thickness （IT）, degree of restenosis （DR）, and VSMC proliferation were observed by electro-microscopic; c-fos, c-myc and proliferation cell nuclear antigen （PCNA） were detected by using immunohistochemistry technique. Results The IT, DR, and proliferating index of VSMC and expression of c-fos, c-myc, PCNA in the experimental group were significantly lower than those in the control group （t=8.19-25.19;t′=6.14,7.36;P〈0.01）. Conclusion Transfection of p^21 gene and antisense c-los nucleotide of the vein grafts can inhibit the proliferation of VSMC.

关 键 词：移植物闭塞 血管 基因治疗 转染 

分 类 号：R363[医药卫生—病理学]