Genetic analysis of segregation distortion of mating-type factors in Lentinula edodes  

作　　者：CHENG Shuiming LIN Fanxue XU Xuefeng LI Anzheng LIN Fangcan 

机构地区：[1]State Key Laboratory of Agricultural Microbiology and Institute of Applied Mycology, Huazhong Agricultural University, Wuhan 430070, China [2]National Engineering Research Center For Wheat, Henan Agricultural University, Zhengzhou 450002, China

出　　处：《Progress in Natural Science:Materials International》2005年第8期684-688,共5页自然科学进展·国际材料（英文版）

基　　金：SupportedbyNationalNaturalScienceFoundationofChina(GrantNo.30170024)andtheResearchFundfortheDoctoralProgramofHigherEducationofChina(GrantNo.2002050421)

摘　　要：Analyses of the mating-type ratio of protoplast monokaryons to spores in 28 strains of Lentinula edodes were performed through statistical methods. The results showed that the segregation distortion phenomenon commonly exists in both types of Lentinula edodes, 17 out of the 28 samples did not display the expected 1 ： 1 ratio of protoplast monokaryons; and among them 8 fruiting strains distorted 1：1：1：1 segregation of spores. The degree of distortion in all tested wild strains was significantly lower than that of cultured strains. In fruiting test of positive-negatlve parent, predominant spores were those with the mating type identical to dikaryotic parent, indicating that the predominance of nuclei mainly depends on the mating type of dikaryotic parent. Data from di-mon mating test verified that monokaryons with acceptor nuclear type are present exclusively in a large amount after dedikaryotization, suggesting that the specificities among B factors and some other unknown factors in cytoplasm are probably responsible for the phenomenon of skewed segregation.Analyses of the mating-type ratio of protoplast monokaryons to spores in 28 strains of Lentinula edodes were performed through statistical methods. The results showed that the segregation distortion phenomenon commonly exists in both types of Lentinula edodes, 17 out of the 28 samples did not display the expected 1∶1 ratio of protoplast monokaryons; and among them 8 fruiting strains distorted 1∶1∶1∶1 segregation of spores. The degree of distortion in all tested wild strains was significantly lower than that of cultured strains. In fruiting test of positive-negative parent, predominant spores were those with the mating type identical to dikaryotic parent, indicating that the predominance of nuclei mainly depends on the mating type of dikaryotic parent. Data from di-mon mating test verified that monokaryons with acceptor nuclear type are present exclusively in a large amount after dedikaryotization, suggesting that the specificities among B factors and some other unknown factors in cytoplasm are probably responsible for the phenomenon of skewed segregation.

关 键 词：Lentinula edodes mating-type factors segregation distortion CYTOPLASM 

分 类 号：Q319[生物学—遗传学]