非放射性原位杂交技术检测胃癌组织c-erbB-2和c-myc癌基因扩增的研究  

c-erbB-2 and c-myc expression is related with pathological progression of gastric cancer：results of a non- radioactive in sire hybridization

作　　者：王克强[1] 李雁[2] 晁储璋[1] 韩国新[1] 刘振忠[1] 东野圣伊[1] 吴家锋[1] 史继学[1] 

机构地区：[1]泰山医学院附属医院,泰安市271000 [2]武汉大学中南医院肿瘤科,430071

出　　处：《中华腹部疾病杂志》2005年第10期703-705,共3页Chinese Journal of Celiopathy

摘　　要：目的研究c—erbB—2和c—myc癌基因在胃癌组织中扩增的意义。方法应用非放射性原位杂交技术检测81例胃癌标本中c—erbB—2和c—myc的扩增情况。结果C—erbB—2和c—myc在胃癌中的扩增率分别为50．6％和67．9％。c—erbB—2基因与胃癌组织分化程度、浸润深度及淋巴结转移有关（P〈0．05），分化程度越差、浸润深度越深、淋巴结有转移，c—erbB—2基因表达率越高，而C—myc癌基因与胃癌组织分化程度、浸润深度及淋巴结转移无关。两基因的扩增具有显著的相关性（x^2=7．26，P〈0．01）。结论c—erbB—2和c—myc扩增是胃癌发生过程中的一个重要的生物学因素，且两者具有较好的协同作用。AIM To study the significance of c - erbB - 2 and c - myc oncogene expression in gastric cancer. MEITHODS 81 gastric cancer specimens were studied for c - erbB - 2 and c - myc oncogene amplification using non - radioactive in situ hybridization method.RESULTS The amplification rates for c- erbB- 2 and c- myc were 50.6% and 67.9% ,respectively, and there were significant correlation in the amplification of these two genes（x^2 =7.26, P〈0.01）. CONCLUSIONS The amplification of c-erbB-2 and c - myc may play an important role in gastric cancer development, and these two genes may have synergistic effect.

关 键 词：胃癌 C—erbB—2基因 C—myc基因 原位杂交 

分 类 号：R735.2[医药卫生—肿瘤]