复方红景天对肝纤维化大鼠肝组织Na^+/Ca^(2+)泵、TGF-β1 mRNA表达的影响  

Effects of compound Rhodiola Sachalinensis A Bor on expression of Na^+/Ca^(2+) exchanger and TGF-β1 mRNA in rats with CCl_4-induced liver fibrosis

作　　者：曾维政[1] 吴晓玲[2] 蒋明德[1] 陈晓斌[1] 秦建平[1] 邓桂英[3] 

机构地区：[1]成都军区总医院消化内科,四川成都610083 [2]重庆医科大学第一医院消化内科,重庆400016 [3]成都市第六人民医院胃镜室,四川成都610051

出　　处：《第四军医大学学报》2005年第17期1554-1557,共4页Journal of the Fourth Military Medical University

基　　金：成都军区"十五"医药卫生科研基金(01A009)

摘　　要：目的:探索复方红景天抗实验性大鼠肝纤维化的疗效与可能的分子机制.方法:用CC l4皮下注射法诱导SD大鼠肝纤维化模型,同时给予复方红景天颗粒口服进行干预性治疗,观察大鼠血清Ⅲ型前胶原、Ⅳ型胶原、透明质酸含量及肝组织Na+/Ca2+泵、TGF-β1,Ⅰ型前胶原α1 mRNA表达水平、肝组织病理学变化.结果:与模型组相比,复方红景天可明显降低大鼠血清PCⅢ(μg/L)(164.25±45.68vs265.54±98.21),CⅣ(96.73±14.68vs159.67±29.64),HA(289.35±75.68vs455.79±113.55)水平,(q=4.26,4.94,3.68,均为P<0.05);复方红景天可抑制NCX,TGF-β1,α1(Ⅰ)mRNA表达(半定量积分分别为1.50±0.19vs2.45±0.31,1.06±0.12vs2.47±0.37,1.26±0.17vs2.50±0.40,q值分别为3.52,3.93,4.04,均为P<0.05).肝组织纤维沉积明显减少(肝纤维化积分为2.43±0.70vs3.53±0.68,q=3.69,P<0.05).NCX,TGF-β1 mRNA表达与PCⅢ,CⅣ,HA,α1(Ⅰ)mRNA及肝组织的纤维化积分呈正相关,相关系数r分别为0.52,0.49,0.56,0.48,0.60及0.47,0.61,0.56,0.49,0.51(均为P<0.05).结论:复方红景天良好的抗肝纤维化作用,其分子机制可能与抑制NCX及TGF-β1mRNA表达从而减少胶原纤维合成有关.AIM： To study the protective effects of compound Rhodiola Sachalinensis A Bor on CCl4-induced liver fibrosis in rats and to investigate the probable molecular mechanisms. METHODS： Male SD rats were divided into 3 groups： normal （N）, compound Rhodiola Sachalinensis A Bor （T） and model （M） groups. The rats in M group and T group were given 300 mL/L CCl4（3 mL/kg, 2 times/wk） for 14 weeks and compound Rhodiola Sachalinensis A Bor （0.5 g/kg, po, daily for 14 weeks） was administered to rats in T group. The serum concentration of PCⅢ, CⅣ and HA in rat was assayed with RIA method and the liver tissue sections were assessed by HE, VG and Masson staining methods. The expression of Na^＋/Ca^2＋ exchanger （NCX） mRNA, transforming growth factor beta 1 （TGF-β1） mRNA and α1 （ Ⅰ） procollagen mRNA in each group were observed with semi-quantitative in situ RT-PCR. RESULTS： Compared with those in M group, the serum PC Ⅲ, C Ⅳ and HA levels in T group markedly decreased by the end of experiment （ 164.25 ± 45.68 vs 265.54 ±98.2, 96.73 ±14.68 vs 159.67±29.64, 289.35 ±75.68 vs455.79 ±113.53, respectively, q = 4.26,4.94, 3.68, P 〈 0.05 ）. The expression of NCX mRNA, TGF-β1 mRNA and α1 （ Ⅰ ） mRNA in liver tissues was obviously inhibited and their semiquantitative scores increased （ 1.50 ± 0. 19 vs 2.4.5± 0.31, 1. 06 ± 0. 12 vs 2.47 ± 0.37, 1. 26 ±0. 17 vs 2.50 ± 0.40, respectively, q=3.52, 3.93, 4.04, P 〈 0.0.5 ）. The semiquantitative scores of hepatic fibrosis staging in rats liver samples also decreased significantly （ mean score： 2.43 ±0.70 vs 3.53 ± 0.68, q = 3.69, P 〈 0.0.5 ）. CONCLUSION. Compound Rhodiola sachalinensis A Bor is effective in protecting against CCl4-induced liver fibrosis in rats. The protective effects may be related to the reduction of collagen production by inhibiting the expression of NCX mRNA, TGF-β1 mRNA and α1 （ Ⅰ ） mRNA.

关 键 词：复方红景天 肝纤维化 Na^+/Ca^2+泵:转化生长因子-β1 肝纤维化大鼠 TGF-Β1 MRNA表达 肝组织纤维 Ca^2+泵 NA^+ 肝纤维化模型 

分 类 号：R575.2[医药卫生—消化系统] R734.2[医药卫生—内科学]