Ibrolipim抗猪动脉粥样硬化的作用  

Effects of ibrolipim against atherosclerosis in minipigs

作　　者：席守民[1,2] 唐朝克[3] 易光辉[3] 廉馨[3] 王佐[3] 张秋菊[2] 尹卫东[2] 

机构地区：[1]河南科技大学医学院,河南洛阳471003 [2]南华大学生命科学与技术学院生物化学与分子生物学教研室 [3]南华大学医学院心血管病研究所,湖南衡阳421001

出　　处：《中国病理生理杂志》2005年第9期1686-1690,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30370675);日本大制药厂新药研究基金资助项目(No.2001-8-28)

摘　　要：目的:探讨合成化合物NO-1886(ibrolipim)调节贵州小香猪脂代谢异常及其抗动脉粥样硬化(AS)的作用机制。方法:贵州小香猪15头,按体重随机分为3组。分别喂普通猪饲料(CD);高脂高蔗糖饲料(HFSD)和高脂高蔗糖饲料加1%ibrolipim。每月末采集血样,测定血脂浓度。第8个月末处死动物,用高效液相色谱(HPLC)分析血浆高密度脂蛋白中胆固醇酯的含量;苏丹Ⅳ染色观察主动脉脂纹的面积;油红O染色观察脂质在肝组织中的沉积。结果:HFSD组血浆总胆固醇(TC)、甘油三酯(TG)和游离脂肪酸(FFA)浓度均高于对照组,主动脉可见脂纹,内膜增厚,内皮细胞损伤,内膜下有大量泡沫化细胞,肝细胞胞浆内大量脂质沉积;ibrolipim组血浆高密度脂蛋白胆固醇(HDL-C)水平高于对照组(其中胆固醇酯高于对照组,TG和FFA浓度低于对照组),肝细胞油红O染色见少量脂质沉积。结论:Ibrolipim可能主要是通过降低血浆TG及升高HDL-C发挥其抗AS作用。AIM： To explore the application mechanism of NO- 1886 （ibrolipim）, a synthetic compound, improving dyslipidemia and inhibiting atherosclerosis in Guizhou minipig,; fed with high fat/high sucrose diet. METHODS： Fifteen Chinese Guizhon minipigs were randomized into three groups with similar body weight [ （ n = 5 in normal control group （CD） ; n = 5 in high fa/high sucrose group （HFSD） ;n = 5 in high fat/high sucrose supplemented ibmlipim group （ HFSD ＋ ibmlipim） ]. Blood samples were withdrawn from the evehole sinus venosus of the animals at the end of each month after fasting overnight. The animals were sacrificed at tile end of 8 months. The concentrations of cholesterol ester in plasma HDL were analyzed by HPLC. The aortic fatty streak- lesions were quantified following lipid staining with Sudan IV. Lipid droplets in liver were observed by Oil red O staining. RESULTS： Compared wilh CD, fasling plasma TC, TG and FFA levels of HFSD were elevated significantly. The aortic fatty streak - lesions were clearly prcsenled in the animals＇ aortas. The intima became rougher and thicker. A lot of lipoid foam cells migrated to regions of intinm and smooth,lh muscle cells, which asseciated with the injuries of internal elastic lamina. Extensive fat deposited in the liver were. observed. Supplementing of 1.0% ibrolipim into high fat/high sucrose diet induced the decrease in plasma TG and FFA concentrations and an increase in plasma HDL- C concentration compared with HFSD. A little fat deposited in the liver were ohserved. CONCLUSION： ibrolipim prevents AS in high fat/high sucrose diet feeding minipigs through decreasing the plasma TG and elevating file plasma HDL- C.

关 键 词：甘油三酯类 脂蛋白类 HDL胆固醇 动脉硬化 猪 Ibrolipim 

分 类 号：R363[医药卫生—病理学]