乌司他丁对失血性休克大鼠的保护作用及其机制的研究  

Protective effect and mechanism of ulinastatin on rats with hemorrhagic shock

作　　者：蒋龙元[1] 杨炼红[2] 钟娃[1] 何志捷[1] 常建星[1] 余涛[1] 王彤[1] 黄子通[1] 

机构地区：[1]中山大学附属第二医院急诊科,广东广州510120 [2]中山大学附属第二医院神经科,广东广州510120

出　　处：《中国病理生理杂志》2005年第9期1701-1704,共4页Chinese Journal of Pathophysiology

基　　金：广东省科技计划项目(No.2004B30601001)

摘　　要：目的:探讨乌司他丁对失血性休克大鼠的保护作用及其机制。方法:采用前瞻对照研究,用Chaudry方法(略作改动)复制大鼠失血性休克模型,60min后回输血液和生理盐水进行复苏,部分加用乌司他丁治疗。检测不同时相的血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、丙二醛(MDA)及超氧化物歧化酶(SOD)的表达水平。结果:大鼠失血性休克与复苏后血清TNF-α、IL-6及MDA含量均明显升高,SOD含量减少。用乌司他丁治疗后大鼠的血压、心率明显改善;血清TNF-α、IL-6及MDA含量均明显下降,SOD含量无明显变化。结论:乌司他丁通过抑制细胞因子的生成、减少氧化损伤而对失血性休克大鼠起保护作用。AIM： To investigate the protective effect of ulinastatin on rats with hemorrhagic shock. METHODS： A prospective, controlled animal study was designed. The model of hemorrhagic shock in rats was produced by Chaudry method. After 60 min, rats were resuscitated by transfusion of shed blood and normal saline, but a half of them were treated with ulinastatin. At different time points after reperfusion, the levels of tumor necrosis factor - alpha （TNF - α）, interleukin - 6 （ IL - 6）, malondialdehyde （MDA） and superoxide dismutase （SOD） in serum were detected. RESULTS： The levels of TNF- α, IL- 6 and MDA significantly increased and the activity of SOD decreased. In the ulinastatin - treated groups, the blood pressure and heart rate were obviously improved ; the levels of TNF - α, IL - 6 and MDA significantly decreased and the activity of SOD had little change after hemorrhagic shock and reperfusion, CONCLUSION： Ulinastatin has a protection effect on rats with hemorrhagic shock by suppressing the production of inflammatory factors and reducing oxidative damage.

关 键 词：乌司他丁 失血性休克 肿瘤坏死因子-α 白介素-6 丙二醛 超氧化物歧化酶 

分 类 号：R363[医药卫生—病理学]