胃癌细胞凋亡与增殖的细胞转换与生存的关系  

Measures of cell turnover (apoptosis and proliferation) and their association with survival in gastric cancer

作　　者：陈金明[1] 周海波[1] 张梁[1] 李伟华[2] 

机构地区：[1]浙江大学医学院附属第二医院外科,浙江杭州310009 [2]福建省立医院外科,福建福州350000

出　　处：《中国病理生理杂志》2005年第9期1758-1762,共5页Chinese Journal of Pathophysiology

摘　　要：目的:探讨胃癌细胞中凋亡与增殖的细胞转换(AI/PI)的变化以及与生存的关系。方法:应用流式细胞仪,分别定量分析细胞的凋亡群体(AI)和增殖群体(PI);共聚焦显微镜对细胞增殖进行形态学观察;Kaplan-Mi-er法分析生存率。结果:肿瘤细胞凋亡与增殖经Pearson检验两者呈正相关,相关系数r=0.58(P<0.01)。在肿瘤细胞的临床病理特征中,AI/PI总体呈下降的趋势(与淋巴转移、远处转移相关,P<0.05)。胃癌细胞AI/PI的表达与肿瘤患者不同存活时间之间无显著差异。根据AI/PI的中位数值0.245,将胃癌细胞分为>0.245组和<0.245组。结果显示两组Kaplan-Meier生存曲线经Log-rank检验无显著差异(P>0.05)。共聚焦显微镜显示肿瘤组织中Ki-67高表达。结论:肿瘤细胞凋亡与增殖密切相关;随着肿瘤细胞的恶性进展,AI/PI总体呈下降的趋势;AI/PI不是评价预后的指标。AIM： To investigate the ratio of apoptotie index and proliferative index （AI/PI） in gastric cancer and their association with survival. METHODS： The apoptotic population and the proliferative population were quantitatively analyzed by flow cytometry. The proliferative cells were identified by confocal microscope and Kaplan - Meier method was used to analyze survival. ~TS ： AI and PI were strongly correlated with a Pearson coefficient of 0.58 （ P 〈 0.01 ）. A stepwise decrease trend in AI/PI was noted in clinical pathological variables, including lymph node metastasis and beyond metastasis （ P 〈 0.05 for each）. No statistically significant difference in AI/PI expression based on different survival times was observed. Using the median AI/PI of 0.245, the cells were divided into two groups （greater than and less than the median AI/PI）. Using Kaplan - Meier survival curves and the Log - rank test, no statistic difference was found in two groups. The high expression of Ki - 67 in tumor was found by confocal microscope. CONCLUSION： AI and PI are. significantly correlated. A stepwise decrease trend in AI/PI may exist in tumor progression. AI/PI mav not he good as a prognostic factor for cancer evaluation.

关 键 词：胃肿瘤 细胞凋亡 细胞增殖 生存 流式细胞术 

分 类 号：R363[医药卫生—病理学]