抗氧化剂和NF-κB对大肠癌细胞IL-8表达的作用及机制  

Effects of antioxidant and NF-κB on the induction of IL-8 in human colon cancer cell line HT-29 cells in vitro

作　　者：周文鹏[1] 吴金民[1] 张行[1] 潘宏铭[1] 方勇[1] 曹厚军[1] 王宏[1] 张俊平[1] 张在云[1] 

机构地区：[1]浙江大学医学院附属邵逸夫医院临床医学研究所,浙江杭州310016

出　　处：《中国病理生理杂志》2005年第9期1779-1782,共4页Chinese Journal of Pathophysiology

摘　　要：目的:探讨NF-κB在大肠癌细胞IL-8诱导表达中的作用及抗氧化剂对大肠癌细胞IL-8诱导表达的影响及其机制。方法:IL-8mRNA表达采用逆转录/聚合酶链反应(RT/PCR)检测,培养上清IL-8蛋白含量用ELISA检测,EMSA法测定细胞核内NF-κB结合活性。结果:抗氧化剂可阻断大肠癌细胞培养体系TNF-α诱导的IL-8生成及IL-8mRNA的表达,TNF-α可诱导大肠癌细胞NF-κB的激活并被抗氧化剂阻断。结论:TNF-α诱导的大肠癌细胞IL-8基因和蛋白表达依赖于NF-κB的激活;抗氧化剂可通过抑制NF-κB的活化而阻断IL-8基因和蛋白的诱导表达。AIM- To investigate the role of nuclear factor κB （NF-κB） in the induction of IL- 8 gene by TNF- α in colon cancer cells and the effect of antioxidant on the induction of IL - 8. METHODS： ELISA was used to detect the concentrations of IL - 8. IL- 8 mRNA was analyzed by using RT - PCR. NF-κB in the cell nuclei was detected with electrophoretic mobility shift assay. RESULTS： （ 1 ） IL - 8 production and IL - 8 mRNA expression induced by TNF-α was blocked by pyrrolidine dithiocarbamate （PDTC）. （2） TNF-α triggered the activation and translocation of NF-κB and PDTC inhibited the activation of NF-κB induced by TNF-α. CONCLUSION： The induction of IL - 8 gene and protein by TNF-α is dependent on the activation of NF-κB. Antioxidants may inhibit the induction of IL- 8 gene and protein through inhibiting NF-κB activation.

关 键 词：结肠肿瘤 白细胞介素-8 NF-ΚB 二硫氨基甲酸吡啶 

分 类 号：R735[医药卫生—肿瘤]