和厚朴酚与厚朴酚在缓解大鼠吗啡戒断反应中对β-内啡肽的影响(英文)  

Effects of honokiol and magnolol on beta-endorphin in relieving morphine withdrawal symptoms in rats

作　　者：黄德彬[1] 余昭芬[2] 李权文[2] 

机构地区：[1]湖北民族学院医学院药理学教研室,湖北省恩施市445000 [2]湖北民族学院医学院外国语学院,湖北省恩施市445000

出　　处：《中国临床康复》2005年第32期230-231,共2页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省教育厅重大基金项目(2000Z09003);2002年国家药品监督局特殊药品研制项目(TYL20020104)~~

摘　　要：背景:现代药理证实和厚朴酚与厚朴酚具有中枢抑制作用和肌肉松弛作用,且有报道证实其可缓解动物吗啡依赖戒断症状。目的:探讨和厚朴酚与厚朴酚在缓解吗啡戒断反应中对β-内啡肽的影响。设计:随机对照实验。单位:湖北民族学院医学院药理学教研室。对象:实验于2003-04-13/29进行,取成年雄性SD大鼠100只,其中30只大鼠作为对照组随机分成生理盐水组、和厚朴酚组和厚朴酚组各10只,另外70只大鼠作为吗啡依赖组,随机分成生理盐水组,和厚朴酚5,40,80mg/kg组与厚朴酚5,40,80mg/kg组7组,各10只。方法:对照组中3个亚组分别腹腔注射生理盐水0.2mL、和厚朴酚与厚朴酚各80mg/kg。吗啡依赖组大鼠逐日增量皮下注射吗啡6d,建立急性吗啡依赖大鼠及吗啡自然戒断模型,在第6天9:00最后一次给吗啡后,10:30腹腔注射给药,生理盐水组给予生理盐水0.2mL,其他6组分别给予和厚朴酚、厚朴酚各5,40和80mg/kg。于11:00分别观察吗啡依赖组每只大鼠1h内各项自然戒断症状。主要观察指标:①各组大鼠脑脊液中β-内啡肽水平。②比较吗啡依赖组中7个亚组大鼠自然戒断症状评分。结果:①脑脊液中β-内啡肽水平:对照组中和厚朴酚与厚朴酚组显著高于生理盐水组(P<0.01),而且和厚朴酚强于厚朴酚(P<0.05)。吗啡依赖+生理盐水组明显低于对照+生理盐水组(P<0.01)。吗啡依赖组中和厚朴酚与厚朴酚5,40,80mg/kg组均高于生理盐水组(P<0.01),而且呈量效关系。②吗啡依赖组大鼠自然戒断症状评分:和厚朴酚与厚朴酚各剂量组湿狗样抖动、舔阴,逃避症状得分均低于生理盐水组(P<0.05,0.01),且剂量越大效果越显著;和厚朴酚与厚朴酚40,80mg/kg组咬牙、扭体及体质量丢失显著低于生理盐水组(P<0.01)。结论:和厚朴酚与厚朴酚可明显抑制吗啡戒断反应,且抑制效应呈量效关系,这一抑制效应与脑内β-内啡肽的增加有关。这�BACKGROUND： It has been verified pharmacologically that honokiol （HL） and magnolol （ML） have distinct effects on central inhibition and muscle relaxation, and some reports prove that they can relieve morphine withdrawal symptoms in animals. OBJECTIVE： To explore the effects of HL and ML on β-endorphin （β- EP） in relieving morphine withdrawal symptoms in rats. DESIGN： Randomized controlled study. SETTING： Pharmacological Department, the Medical School of Hubei national College. PARTICIPANTS： The experiment was conducted from 13 to 29 April, 2003. Among 100 adult male SD rats, 30 rats as control group were randomly divided into three subgroups： saline group, HL group and ML group with 10 in each group. The other 70 rats as morphine-dependent group were divided into normal saline group, 5, 40 and 80 mg/kg HL and ML groups with 10 in each group. METHODS： 0.2 mL normal saline, 80 mg/kg HL and 80 mg/kg ML were injected intraperitoneally into rats in control group. The dose of morphine subcutaneously injected was increased in the rats in morphine-dependent group for 6 days to establish the model of acute morphine dependence and model of natural morphine withdrawal symptoms. The last injection was performed at 9：00 on the morning of day 6, and the intraperitoneal injection was performed at 10：30 on the same morning. 0.2 mL normal saline was injected into the rats of saline group. 5, 40 and 80 mg/kg HL and 5, 40 and 80 mg/kg ML were given to rats in the other six groups, respectively. Starting from 11：00, half an hour later, all natural morphine withdrawal symptoms were observed in rats within an hour. MAIN OUTCOME MEASURES：① The amount of β-EP was assayed in each group. ② Scores of natural morphine withdrawal symptoms were compared among rats in the 7 morphine-dependent subgroups. RESULTS：①β-EP level in CSF： It was obviously higher in HL, ML and control groups than in normal saline group （P 〈 0.01）, and HL group had higher β-EP level than ML group （P 〈 0.05）. �

关 键 词：吗啡依赖 和厚朴酚 厚朴酚 Β内啡肽 

分 类 号：R749.1[医药卫生—神经病学与精神病学]