补肾益智方拆方含药血清对阿尔茨海默病细胞模型的作用(英文)  

Effects of drug serum in broken bushen yizhi formulas on cell model of Alzheimer disease

作　　者：陈云波[1] 赖世隆[1] 胡镜清[1] 王奇[1] 程淑意[1] 

机构地区：[1]广州中医药大学DME中心,广东省广州市510405

出　　处：《中国临床康复》2005年第32期250-253,共4页Chinese Journal of Clinical Rehabilitation

基　　金：国家"九五"攻关项目资助(96-906-0902)~~

摘　　要：背景:中药补肾益智方对阿尔茨海默病大鼠模型学习记忆能力有一定的保护作用,该方含药血清也能减轻神经瘤细胞NG108-15细胞对β淀粉样蛋白的神经毒性反应,为了进一步了解该方的作用机制及其配伍规律,需对该方进行拆方研究。目的:研究补肾益智方拆方亚组的含药血清对阿尔茨海默病细胞模型生长、分化等方面的影响,从血清药理学的角度探讨该方的配伍规律。设计:随机对照实验。单位:广州中医药大学临床药理研究所DME中心。对象:实验于2003-01/08在广州中医药大学临床药理研究所DME中心实验室进行。实验对象为3月龄SD雄性健康大鼠40只和冻存的NG108-15细胞株。方法:①制备药物血清:将40只SD大鼠随机分为对照组、原方组(蛇床子、枸杞子、人参、何首乌、丹皮、冰片)、补肾组(蛇床子、枸杞等)、益气养血组(人参、制首乌等)、去冰片组(蛇床子、枸杞子、人参、何首乌、丹皮),每组8只。将各组中药浓缩液按按10μL/g(相当于生药6g/kg)分别进行灌胃,连续1个月,对照组灌等量生量盐水。各组大鼠最后1次灌胃2h,麻醉后心脏采血,分离血清备用。②细胞增殖、培养及分化:将体外培养的NG108-15细胞分为6组,对照组、模型组孔中的增殖培养液含正常大鼠血清,其余4组分别为原方组及3个亚组的大鼠含药血清;同时每个孔加淀粉样β蛋白25～35至终浓度5umol/L(对照组除外),继续培养48h。主要观察指标:用MTT法测定细胞增殖数和存活率;同时检测分化突起细胞占总细胞数的比率及突起平均长度。结果:①细胞增殖情况:模型组A值显著低于对照组(0.520±0.022,0.665±0.037,P<0.01),各含药血清组A值均高于模型组,但以去冰片组效果最显著(0.636±0.035,P<0.01)。②分化细胞存活率:模型组显著低于对照组(58.4%,100%),各含药血清组均高于模型组,但以去冰片组效果最显著(75.8%,P<0.01)。③分化突BACKGROUND：Chinese herb. bushen yizhi formula protects at certain extent learning and memory in rat model of Alzbeimer disease. The drug serum in this formula can alleviate neurotoxie reaction of nerve tumor cell NG 108-15 to beta-amyloid protein. In order to understand further the mechanism and compatibility of the foruta, it is necessary to carry on the study on the broken formulas. OBJECTIVE：To study the effect of drug serum in subgroups of broken bushen yizhi formulas on growth and differentiatiou of cell model of Alzheimer disease and probe into the compatibility rule of bushen yizhi formula in view of serunt pharmacology. DESIGN： Randomized controlled experiment. SETTING： DME Center of Clinical Pharmacological Institute Affiliated to Guangzhou University of Traditional Chinese Medicine. PARTICIPANTS：The experiment was performed in DME Center of Clinical Pharmacological Institute Affiliated to Guangzhou University of Traditional Chinese Medicine from January to August 2003, in which, 40 healthy mate SD rats of 3 months old were employed and NG108-15 cell line was frozen-preserved, METHODS：①Preparation of drug serum： Forty SD rats were randomized into the control, original formula group （No.1 group） [shechuanzi （Cnidium monnieri （L.） Cuss）. gouqizi （Lycium harharum L）, renshen （Panax ginseng C.A.Mey.）, heshouwu （Polygonuro multiflorum Thunb.）, danpi （Paeonia Suffrutieisa Andr.） and bingpian （Borneolum）], kidney replenishment group （No. 2 group） [shechwanzi （Cnidiuro monnieri （L.） Cuss）, gouqizi （Lyeiuro barbaruro L.）. etc.], group for henefiting qi and nourishing blood （No.3 group） [renshen （Panaz ginseng C.A.Mey）. zhishouwu （Polygonum multiflorum Thunb.）. etc.] and group with bingpian （Borneolum） removed （No.4 group） [Panax ginseng C.A.Mey.]. heshouw u （Polygonum multiflorum Thunb.） and danpi （Paeonia Suffruticisa Andr.）], 8 rats in each group. The concentrated Chinese herbal solutions of every.group were applied at 1

关 键 词：阿尔茨海默病/中药疗法 淀粉样Β蛋白 补肾药 

分 类 号：R749.1[医药卫生—神经病学与精神病学]