Study on the Interaction between Strychnine and Bovine Serum Albumin by Capillary Electrophoretic Frontal Analysis  

作　　者：Shao Ping FU Xiao Zhe ZHANG Feng ZHANG Qing XU Hong Bin XIAO Xin Miao LIANG 

机构地区：[1]Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023

出　　处：《Chinese Chemical Letters》2005年第9期1233-1236,共4页中国化学快报（英文版）

基　　金：The authors are grateful to the National Natural Science Foundation of China(Grant No.20235020 and 20372062)for financial support.

摘　　要：The protein binding constant, binding sites of the Strychnos alkaloid-strychnine and bovine serum albumin （BSA） was determined by capillary electrophoretic frontal analysis （CE-FA） for the first time. The experiment was carried out in a polyacrylamide-coated fused silica capillary （48.4 cm×50 μm i.d., 38.1 cm effective length） with 20 mmol/L citrate/MES buffer （pH 6.0, ionic strength 0.17）. The applied voltage was 12 kV and detection wavelength was set at 257 nm. The plateau height of the peak was employed to determine the unbound concentration of drug in BSA equilibrated sample solution based on the external drug standard in the absence of protein. The present method provides a convenient, accurate technique for the early stage of drug screening.The protein binding constant, binding sites of the Strychnos alkaloid-strychnine and bovine serum albumin （BSA） was determined by capillary electrophoretic frontal analysis （CE-FA） for the first time. The experiment was carried out in a polyacrylamide-coated fused silica capillary （48.4 cm×50 μm i.d., 38.1 cm effective length） with 20 mmol/L citrate/MES buffer （pH 6.0, ionic strength 0.17）. The applied voltage was 12 kV and detection wavelength was set at 257 nm. The plateau height of the peak was employed to determine the unbound concentration of drug in BSA equilibrated sample solution based on the external drug standard in the absence of protein. The present method provides a convenient, accurate technique for the early stage of drug screening.

关 键 词：STRYCHNINE binding constant capillary electrophoretic frontal analysis bovine serumalbumin 

分 类 号：O629.73[理学—有机化学]