贵农001中抗白粉病基因的RAPD标记研究  

RAPD markers linked to the powdery mildew resistance gene in wheat line Guinong 001

作　　者：徐如宏[1] 任明见[1] 思彬彬[1] 张超[1] 张庆勤[1] 

机构地区：[1]贵州大学麦作研究中心,贵州贵阳550025

出　　处：《山地农业生物学报》2005年第4期283-286,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省"十五"攻关资助项目[15001A-9;(2001)1112];贵州省优秀科技教育人才省长专项资金项目

摘　　要：运用RAPD技术,采用分离群体分组分析法(BSA)对小麦种质贵农001中的抗白粉病基因进行了分子标记研究。结果表明,引物S2018可在抗病亲本贵农001和抗病单株中扩增出特异的DNA片段,而在感病材料和感病亲本龙96-6239中不能扩增出同样的DNA片段,该特异DNA片段分子长度约为900bp。用F2分离群体(110株植株)进行遗传连锁性分析,引物S2018扩增的特异DNA片段与贵农001抗白粉病基因相连锁,其遗传距离为1.7 cM。该标记的获得为将贵农001中抗白粉病基因向其它小麦育种材料的转移提供了有效的选择手段。Random Amplified Polymorphic DNA （RAPD） and BSA method were employed to detect molecular markers linked to powdery mildew resistance gene in wheat line Guinong 001. The results showed the DNA fragments were amplified in the resistant parent Guinong 001 and resistant materials by one primer S2018, but the fragments were not amplified in the susceptible materials and susceptible parent long 96 - 6239. The DNA fragment amplified by the primer S2018 was about 900bp. The genetic linkage of the DNA fragment by the primer S2018 with powdery mildew resistance gene was tested on a Segregating F2 population （110 plants）. The result showed that the DNA fragment was closely linked to the powdery mildew resistance gene in wheat line Guinong 001. In which the genetic distance between S2018 and powdery mildew resistance gene was 1.7cM. The markers acquired would offer an effective elected means to transfer the powdery mildew resistance gene in wheat Guinong 001 to other wheat materials.

关 键 词：小麦 白粉病 抗性基因 分子标记 随机扩增多态性 

分 类 号：S512.102[农业科学—作物学] Q943.2[生物学—植物学]