大鼠脑血栓模型的实验研究  

Experiment research of brain thrombosis model in rat

作　　者：刘瑜[1] 胡文立[2] 宋爱利[1] 刘国贞[1] 许兰萍[2] 卢玲巧[3] 杨慧[1] 董小黎[1] 

机构地区：[1]首都医科大学基础医学院病理教研室,100054 [2]首都医科大学附属朝阳医院神经内科,100020 [3]首都医科大学病理生理教研室,100054

出　　处：《实用医学杂志》2005年第18期1992-1993,共2页The Journal of Practical Medicine

摘　　要：目的:通过实验建立能快速评价溶栓药疗效并与临床接近的实验动物模型。方法:应用导管从大鼠颈外动脉逆行至颈总动脉,注入已形成血栓。于手术后3、6、10、18h观察行为障碍并评分,分别取脑观察梗死灶及脑组织病理改变。结果:该模型行为障碍和脑梗死体积较为恒定,光镜下6h脑组织神经细胞开始肿胀,10h、18h病变严重。结论:该模型简便易行,对动物损伤小,重复性较好,可为脑缺血发病机制研究及临床脑血栓的治疗研究提供一种有效手段。Objective To establish an experiment animal model of brain thrombus that can evaluate effect of thrombelysis drugs and adjacent to clinic. Methods Twenty-four Wistar rats were employed in this study. A cather was introduced into the external carotid artery （ECA） lumen into the lumen of internal carotid artery （ICA）, and then injected the formed thrombosis. Behavior disorders were observed at 3, 6, 10, 18 h after the operation. Infarction and histopathologic changes were examined respectively. Results The model had stable behavior disorder score and infarct volume. Nerve cell swelled at 6 h after the operation, degeneration and necrosis of nerve ceil were severe. Conclusion The model has the advantages of easy operation, little damage to animal, preferable repetition character. It may be offer an available means for research about mechanism of brain ischemia and therapy of clinic brain thrombosis.

关 键 词：大鼠 脑血栓 动物模型 发病机制 纤维蛋白 

分 类 号：R-332[医药卫生]