蚓激酶白蛋白纳米粒中的酶活力测定  

Determination of biological activity of lumbrokinase-albumin nanoparticles

作　　者：王玮[1] 尹宗宁[1] 李铜铃[1] 李毓琦[1] 邹敬伟[1] 

机构地区：[1]四川大学华西药学院,成都610041

出　　处：《中国新药杂志》2005年第8期1019-1021,共3页Chinese Journal of New Drugs

摘　　要：目的:测定蚓激酶白蛋白纳米粒中蚓激酶的活力。方法:以对甲基苯磺酰-L-精氨酸甲酯盐酸盐(TAME)为底物测定蚓激酶的活力,通过载药量与包封率2个指标进行质量评价。结果:白蛋白测定方法的RSD均小于0.3%,高、中、低浓度平均回收率分别为(99.47±0.93)%,(98.51±1.42)%,(98.69±2.43)%;蚓激酶测定方法的高、中、低浓度的RSD分别为0.62%,0.37%,1.82%,平均回收率分别为(99.25±1.98)%,(97.61±1.13)%,(97.15±2.21)%。蚓激酶白蛋白纳米粒的载药量为(4.84±0.41)%,包封率为(84.14±3.41)%。结论:通过测定蚓激酶白蛋白纳米粒中蚓激酶活性和白蛋白的含量,确定蚓激酶载药量与包封率,所建立的酶学测定方法快速简便、适用可行。Objective： To determine the biological activity of lumbrokinase in lumbrokinase-albumin nanoparticles. Methods：The biological activity of lumbrokinase was determined by the method of TAME and the quality of nanoparrticles was evaluated by encapsulation efficiency and drug loading. Results. RSD of determinations of bovine serum albumin（BSA） were all lower than 0.3 %, the mean recovery were （99.47 ± 0.93） %, （98.51 ± 1.42） %, （98.69 ± 2.43） %, respectivly, for high, middle, low concentration of BSA. RSD of determinations of lumbrokinase were 0.62 %, 0.37 %, 1.82 %, the mean recovery were （99.25 ± 1.98） %, （97.61 ± 1.13） %, （97.15 ± 2.21 ） %, respectively, for high, middlle, low concentration of nanoparticles. The drug loading rate of nanoparticles was （4.84 ± 0.41 ） % and the entrapment rate was （84.14 ± 3.41 ） %. Conclusion： The encapsulation efficiency and drug loading of nanoparticles was determined by those quantitative method and the methods are suitable for this study.

关 键 词：牛血清白蛋白 蚓激酶 纳米粒 酶活力 紫外分光光度法 

分 类 号：R917.2[医药卫生—药物分析学] R944.9[医药卫生—药学]