兔关节盘前移位后髁突软骨细胞中OPG和OPGL的表达及意义  

OPG and OPGL expression in condyle cartilage of temporomandibular joint following anterior disc displacement

作　　者：周艺群[1] 胡济安[2] 谷志远[1] 李怡宁[2] 

机构地区：[1]浙江大学医学院附属口腔医院口腔颌面外科,浙江杭州310006 [2]浙江大学医学院附属口腔医院口腔病理科,浙江杭州310006

出　　处：《上海口腔医学》2005年第4期374-377,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30471900)

摘　　要：目的:探讨关节盘前移位后髁突软骨中护骨素(osteoprotegerin,OPG)及其配体(osteoprotegerinligand,OPGL)表达的变化及意义。方法:20只日本大耳白兔,16只用弹力丝牵引关节盘前伸部,建立颞下颌关节盘前移位的动物模型,分别于术后1、2、4、8周处死。2只行模拟手术作为手术对照组,术后2周处死;另2只不行手术,作为空白对照。取颞下颌关节标本,HE染色,观察其镜下结构,SP免疫组化法检测髁突软骨中OPG和OPGL的表达与分布,并对各标本OPG和OPGL的表达进行灰度测定。用单因素方差分析法对所得数据进行统计学分析。结果:各实验组动物术后体重无明显减轻,伤口愈合良好,大体及显微镜下观察,关节盘明显前移位。OPG和OPGL在各组髁突软骨的增殖层及肥大层浅层均有表达,其中关节盘移位组OPG、OPGL染色较强,染色灰度值与对照组均有显著性差异(P<0.05),但OPG/OPGL比率基本稳定。结论:关节盘移位后OPG和OPGL的表达均有升高,可能参与关节盘移位后髁突软骨病变的发展及转归。PURPOSE： To investigate the change of osteoprotegerin （OPG） and OPGL expression and its significance in condylar cartilage after anterior disc displacement （ADD）. METHODS： Twenty healthy adult Japanese rabbits （from medical animal center of Zhejiang University） weighing from 2 to 3 kg were used in this study. Sixteen rabbits were suhjected to surgical anterior disc displacement in the right temporomandibular joints （TMJ）, four rabbits were sacrificed 1, 2, 4, 8 weeks post-operation,respeetively; two rabbits with sham-operation and two without operation were as control. The structure of temporomandibular joint was observed under microscope. The expression of OPG and OPGL in condylar cartilage were examined by S-P immunohistochemical staining and measured with Image Computer Analysis Software. Date was statistically analyzed by one-way ANOVA. RESULTS： Experimental and sham-operative animals tolerated the surgical procedures well. The discs displaced anteriorly were observed in the surgical rabbits by general and microscope observation. The expression of OPG and OPGL was detected in the proliferating zone and hypertrophy zone cells of condyle in all animals. However, the expression of OPG and OPGL was stronger in the surgical group. There was significant difference in average gray degree （P〈0.05）between the experimental and control groups. The ratio of OPG/ OPGL was stable. CONCLUSIONS： The expression of OPG and OPGL in condylar cartilage was increased after ADD. They might play a rote in the development and prognosis of the temporomandibular condylar cartilage alteration after ADD.Supported by National Natural Science Foundation of China （30471900）.

关 键 词：护骨素 护骨素配体 关节盘移位 颞下颌关节 髁突 

分 类 号：R782[医药卫生—口腔医学]