Se(IV)与藻蓝蛋白相互作用的谱学研究  

Spectroscopic studies on interaction of Se(IV) with phycocyanin

作　　者：郭振江[1] 杨芳[1] 郑文杰[1] 白燕[1] 

机构地区：[1]暨南大学化学系,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2005年第3期390-395,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金(20271022);教育部重点课题(01141);广州市科委基金课题(2001-J-010-01)资助项目

摘　　要：　用吸收、荧光、红外等谱学方法研究了藻蓝蛋白(PC)与Se(IV)的相互作用.结果表明,SeO2-3加入后,藻蓝蛋白在620nm处的特征光吸收减弱,并随Se(IV)浓度和时间的增加而降低,而278和347nm处的光吸收增强;荧光发射和荧光激发也逐渐减弱,而599和629nm处的2个激发峰的相对强度与对照相反.PC在475和662nm处分别出现共振散射峰.Se(IV)与PC的作用后,在595nm处出现强的共振散射峰,被指认为液相纳米硒粒子与PC生成的缀合物的共振散射峰.红外光谱图中,PC的酰胺I带为1653 2cm-1,为α螺旋,而PC-Se(0)生物缀合物的酰胺I带为1647 0cm-1,属无规则卷曲.The reaction of Se（Ⅳ） with Phycocyanin（PC） was investigated by ultraviolet, spectrofluorometry and infrared methods. The results of the absorption spectra showed that the characteristic absorption peak of PC at 620 nm was decreased gradually but the absorption peaks of PC at 278 nm and 347 nm were increased gradually with the increase of time and con- centration of Se（Ⅳ）. Meanwhile, its fluorescence emission peak and two fluorescence excitation peaks all decreased, but the relative fluorescence intensity of excitation peaks at 599nm and 629nm were different from the control. The maximum resonance scattering peak of PC was at 662nm but the maximum peak of production of Se （Ⅳ） with PC was at 595nm, which showed that there was PC - Se（0） bioconjugate. The results of FF-IR showed that amide Ⅰ band of PC （1653.2 cm^-1） was α-helix and that of PC-Se（0）（1647.0 cm^-1） was random coil.

关 键 词：Se(Ⅳ) 钝顶螺旋藻 藻蓝蛋白 光谱 生物缀合物 

分 类 号：Q51[生物学—生物化学]