广西肝病患者及献血员的HCV　Serotype和Genotype的分子生物学研究  

Molecular Biological Research of HCV Serotype and Genotype among Patients with Liver Diseases and Blood Donors in Guangxi,Southern China

作　　者：葛宪民[1] 王树声[1] 李丹亚[1] 黄果勇[1] 李菌[1] 周开姣[1] 钱小燕[1] 潘海东[1] 王缨[1] 章玲珠[1] 王红[1] 沟上雅史 吴蓉蓉 曹坤 大野智仪 哲户悦朗 铃木馨 大羽健一 

机构地区：[1]广西壮族自治区卫生防疫站,广西医科大学,日本名古屋市立大学医学院

出　　处：《广西预防医学》1996年第2期71-79,共9页Guangxi Journal of Preventive Medicine

摘　　要：选择广西不同地市的肝病患者１０３例（ＡＨ８、ＣＡＨ３６、ＣＰＨ３６、ＬＣ７、ＨＣＣ１６）和献血员３５５例进行Ａｎｔｉ－ＨＣＶ阳性率、ＨＣＶＳｅｒｏｔｙｐｅ和Ｇｅｎｏｔｙｐｅ的分子生物学研究。结果表明：肝病患者Ａｎｔｉ－ＨＣＶ阳性１２例（ＡＨ１／８。ＣＡＨ２／３６。ＣＰＨ９／３６。ＬＣＯ／７、ＨＣＣ０／１６），阳性率为１１．６５％，明显高于同室检测兰州和南京肝病患者的Ａｎｔｉ－ＨＣＶ阳性率４．３％和４．２％（Ｐ＜０．０１）；２４０例献血员中检出Ａｎｔｉ－ＨＣＶ阳性１１例（４．５８％），明显低于同室检测兰州献血员的Ａｎｔｉ－ＨＣＶ阳性率３５．００％（Ｐ＜０．０１）；本文两组的Ａｎｔｉ－ＨＣＶ阳性率也存在显著性差异（Ｐ＜０．０１）。肝病患者组的ＨＣＶＳｅｒｏｔｐｙｅ１型、２型、１＋２型和无法定为１和／或２型者分别为３３．３３％、０、０和６６．６７％；而献血员组的ＨＣＶＳｅｒｏｔｙｐｅ分别为６５．９５％、１．１０％、２．２０％和３０．７７％。此外，肝病患者组的ＨＣＶＧｅｎｏｔｙｐｅ均属ｌｂ型（５０．００％）和ｌｂ混合型（５０．００％）；献血员组的ＨＣＶＧｅｎｏｔｙｐｅ中ｌａ型、ｌｂ型、ｌｂ混合型和无法定为１和／或２?Guangxi is well known as ahigh prevalent region of viral hepatitis andhepatocellular carcinoma at home and abroad。However,it is still unknown epidemic situa-tion of henatitis C virus(HCV)and therewere no report about HCV serotype and HCVgenotype.To understand the epidemic situa-tion of HCV infection,HCV serotype andgenotype among patients with liver diseasesand blood donors in Guangxi,103 patientswith several liver diseases(AH 8 cases,CA H36 cases,CPH36 cases,LC 7 cases,HCC 16cases)and 355 blood donors were enrolled inthis study and were determined by anti-NCV,NCV serotype,HCV genotype andHCV sequence,Results showed that among103 patients with liver diseases,12 cases(AH1,CAH 2 and CPH 9)were seropositive of an-ti-HCV and seropositive rate of anti-HCVwere 11.65 percent which was significantlyhigher than among patients with liver diseasesin Nanjing (4.3%)and Lanzhou(4.2%)ofChina(P<0.01).Among 240 persons of 355blood donors,11(4. 58%)were seropositivefor anti-HCV,which was significantly lowerthan among blood donors(35.00%)inLanzhou Western China (P<0.01).In this study,Seropositive rate of anti-HCV amongpatients with liver diseases was significantlyhigher than among blood donors (P<0.01)Among anti-HCV positive samples of pa-tients with liver diseases、we determined theSerotype of all samples.4 33.33%) and 8(66.67%)were HCV Serotypel and unde-cided,respectively.Among 91 anti-HCVpositive samples of 355 blood donors,60(65.93%),1(1.10%),2(2.20%)and 28(30.77%)were HCV Serotype 1,2,1+2 and Un-decided,respectively.Among anti-HCVpositive 12 samples of patients with liver dis-eases,we determined HCV genotype of allsamples by PCR and/or sequence metheds.5(41.67%),1(8.33%)and 6(50. 00%)werethe genotype la+lb mixed,la + lb + 3amixed and lb,respectively.Among 41 casessamples of 91 anti-HCV positive blooddonors,1(2.44%),6 (14.63%),3(7.32%),23(56. 10%),3( 7.32%)and 5(12.20%)were the Genotype la,la+lb mixed,1a+ 1b+3a mixed,16,1b+26and undecid-ed respectively.

关 键 词：肝疾病 献血员 基因型 分子生物学 丙型肝炎病毒 

分 类 号：R575.02[医药卫生—消化系统] R373.21[医药卫生—内科学]