诱导型一氧化氮合酶在末端病大鼠跟腱末端区表达及分布的规律  

Regularity of the expression and distribution of inducible nitric oxide synthase in the achilles tendon terminal region of rats with enthesiopathy

作　　者：王和平[1] 滕宇[2] 胡亚哲[1] 黎明 扈盛[2] 

机构地区：[1]华中师范大学体育系,湖北省武汉市430070 [2]武汉体育学院,湖北省武汉市430079 [3]湖北省直机关医院康复科,湖北省武汉市430071

出　　处：《中国临床康复》2005年第24期159-161,共3页Chinese Journal of Clinical Rehabilitation

基　　金：湖北省自然科学基金项目(2003ABA161)~~

摘　　要：目的:探讨诱导型一氧化氮合酶在末端病大鼠跟腱末端区组织的损伤及适应性改变中的作用。方法:实验于2004-04/07在华中师范大学体育系运动人体科学实验室完成。将8只健康雄性Wistar大鼠随机分为正常对照组2只和造模组6只。采用“电刺激跳跃法”建立大鼠跟腱末端病模型。4周后处死取其双侧后足跟腱末端,用免疫组化方法检测各组诱导型一氧化氮合酶表达,以ImageProPlus5.0图像分析系统测定阳性信号积分光密度值进行评估。结果:纳入动物8只,进入结果分析8只,无脱失值。两组跟腱末端区各部位均有诱导型一氧化氮合酶的表达,跟腱、纤维软骨、钙化软骨、跟骨及腱骨关节面的诱导型一氧化氮合酶积分光密度值表达造模组高于正常对照组(233.16±147.95,2.95±2.80;157.50±88.28,12.98±3.78;212.26±150.21,22.71±15.90;126.06±98.15,13.26±4.57;427.89±288.73,46.67±21.13,P<0.01或P<0.05),而在骨髓腔及腱围区诱导型一氧化氮合酶表达差异不显著(767.62±495.15,466.79±275.08;940.80±596.84,625.98±254.71,P>0.05)。结论:诱导型一氧化氮合酶在末端病大鼠大鼠跟腱末端区各部位均有明显表达,大部分区域和对照组大鼠存在较明显的差异。诱导型一氧化氮合酶在末端病大鼠跟腱末端区的发病中可能起着双重作用,它除了介导组织的损伤过程外还对末端区组织在结构和功能上的适应性改变起着调控作用。AIM： To investigate the effect of inducible nitric oxide synthase on the damage and adaptive changes of the tissue in the achilles tendon terminal region of rats with enthesiopathy. METHODS： The experiment was carried out in the Laboratory of Sports and Human Science, Department of Physical Education, Central China Normal University from April to July 2004. Eight healthy male Wistar rats were randomly divided into the control group （n=2） and the experimental group （n=6）. Rat models of achilles tendon enthesiopathy were established with electrostimulating jumping method. The rats were killed after 4 weeks, and their bilateral achilles tendon terminal regions of hindfoot were taken, and then the expression of inducible nitric oxide synthase was detected with immunohistochemirstry in all the groups, and it was assessed with the positive signal integral optical density detected with the Image Pro Plus 5.0 imaging analyze system. RESULTS： All the 8 rats were involved in the analysis of results without deletion. The expression of inducible nitric oxide synthase was observed in each part of achilles tendon terminal regions in both groups. The values of integral optical density of inducible nitric oxide synthase in achilles tendon fibrous cartilage, calcified cartilage, caleaneus and articular surface of tendon bone were higher in the model group than in the normal control group （233.16±147.95, 2.95±2.80; 157.50±88.28, 12.98±3.78; 212.26±150.21, 22.71±15.90;, 126.06±98.15, 13.26±4.57; 427.89±288.73, 46.67±21.13, P 〈 0.01 or P 〈 0.05）; The expressions of inducible nitric oxide synthase in medullary cavity of bone and the region around tendon were not significantly different between the two groups （767.62±495.15,466.79±275.08; 940.80±596.84,625.98±254.71 ）. CONCLUSION： There are obvious expressions of inducible nitric oxide synthase in each part of achilles tendon terminal regions in rats with enthesiopathy, and those in most regions are obviously different from those of normal rat

关 键 词：跟腱 一氧化氮合酶 一氧化氮 诱导型一氧化氮合酶 WISTAR大鼠 跟腱末端病 表达及 正常对照组 适应性改变 分布 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]