野罂粟碱镇痛作用及其机制研究(英文)  

Analgesic effect of nudicauline and its mechanism

作　　者：刘永平[1] 佟继铭[1] 

机构地区：[1]承德医学院中药研究所,河北省承德市067000

出　　处：《中国临床康复》2005年第24期238-239,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：背景:镇痛是野罂粟主要功效之一。以往的研究证明其具有显著镇痛作用的主要有效部位为总生物碱。野罂粟碱是从其总生物碱中分离出来的一种新的生物碱。目的:探讨野罂粟碱的镇痛作用及其作用机制。设计:以实验动物为观察对象的随机对照实验。单位:承德医学院中药研究所材料:实验于2001-08/2003-12在承德医学院中药研究所(河北省中药研究与开发重点实验室)完成。选用140只小鼠,随机分为14组,每组10只。方法:①镇痛效果比较。随机分5组:盐水对照组,罂粟碱2.5,5.0和10.0mg/kg组和吗啡组。②与内啡肽关系比较。随机分5组:盐水对照组,野罂粟碱组,野罂粟碱+纳洛酮组,吗啡组和吗啡+纳洛酮组。③与一氧化氮关系比较。随机分4组,盐水对照组,野罂粟碱组,野罂粟碱+L-精氨酸400m g/kg组和L-硝基精氨酸甲酯37.5mg/kg组。致痛方法为小鼠右后足掌皮下注射10g/L 甲醛20μL,疼痛观察项目为行走自如得0分;跛行,不动时撑地得1分;抬足得2分;抖足或舔足得3分。观察分两个时相:致痛后0～10m in 为第1时相,20～30m in 为第2时相。主要观察指标:不同药物作用下各组小鼠的疼痛反应评分。结果:①野罂粟碱可显著的降低甲醛致痛小鼠第2时相的疼痛反应评分(P <0.05～0.01)。大剂量对第1时相的疼痛反应也有一定的抑制作用(P <0.05)。吗啡对两个时相的疼痛反应均有显著的抑制作用(P <0.01)。②纳洛酮可完全拮抗吗啡的镇痛作用,而对野罂粟碱的镇痛作用无明显影响。③L-精氨酸可减弱野罂粟碱对第2相疼痛反应的抑制作用,L-硝基精氨酸甲酯可增强野罂粟碱的镇痛作用。结论:野罂粟碱有显著的镇痛作用,阿片拮抗剂纳络酮可完全拮抗吗啡的镇痛作用,但不能改变野罂粟碱的作用,提示野罂粟碱的镇痛作用与内源性阿片系统无关。L-精氨酸可部分的抑制野罂粟碱的镇痛作用,而L-硝基精氨�BACKGROUND：One function of papaver nudicaule L is its analgesic effect.Previous studies showed that total alkaloid,extracted from papaver nudicaule,possesses observable analgesic effects.Nudicauline,separated from the total alkaloid of papaver nudicaule,is a new kind of alkaloid. OBJECTIVE：To study the analgesic effeci of nudicauline and its mechanism of action. DESIGN：A randomized controlled study based on the experimental animals. SETTING：Institute of Traditional Chinese Medicine ,Chengde Medical Colege. MATERIALS：The experiment was conducted in the Institute of Traditional Chinese Medicine of Chengde Medical College （Key Laboratory for Research and Exploitation of Traditional Chinese Medicine） from August 2001 to December 2003.Totally 140 mice were included and randomly divided into 14 groups with 10 mice in each group in this experiment. METHODS： ①Study of the analgesic effect.The animals were randomly assigned to 5 groups：saline control group,and nudicauline 2.5,5.0 and 10.0 mg/kg groups as well as morphine group. ②relation with endor-phin.The animals were divided into 5 groups：saline control group,nudicauline group,nudieauline＋naloxone group,morphine group,and morphine ＋naloxone group.③ relation with nitrogen monoxide.The animals were divided into 4 groups： saline group,nudicauline group,nudicauline ＋L-arginine 400 mg/kg group,and n-nitro-L-arginine methyl ester （L-NAME） 37.5 mg/kg group.Totally 20 μL l0 g/L formalin was injected into the right hindlimbs of the mice.The pain observation item：walking freely was 0 point;being lame,could stand on the floor without moving was 1 point;could lift their feet was 2 point;could shake or lick their feet was 3 points.The observation fell into 2 phases：0 to 10 minutes after pain induction was set as the first phase,and 20 to 30 minutes was set as the second phase. OUTCOME MEASURES：The pain response scores of the mice in each group under the effect of different drugs. RESULTS： ①Nudicauline reduced significantly the score

关 键 词：镇痛 野罂粟碱 吗啡 精氨酸 一氧化氮 

分 类 号：R749.61[医药卫生—神经病学与精神病学]