珊瑚藻藻红蛋白β亚基脱辅基蛋白基因克隆与序列分析  

Cloning and analysis of beta subunit sequence of phycoerythrin gene from Corallina officinalis

作　　者：王盛[1] 钟伏弟[1] 吴祖建[1] 林奇英[1] 谢联辉[1] 

机构地区：[1]福建农林大学植物病毒研究所

出　　处：《福建农林大学学报（自然科学版）》2005年第3期334-338,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省科技厅资助项目(200H004和2001Z127)

摘　　要：首次报道珊瑚藻R-藻红蛋白β亚基基因的全序列.测定的基因序列长为983个碱基,包括β亚基起始密码子上游的10个碱基、β亚基编码区的534个碱基(编码177个氨基酸)、101个碱基的间隔区以及α亚基编码区近5′端的338个碱基(编码112个氨基酸).对其序列在核酸和氨基酸水平上的同源性与已知基因序列的9种红藻作了比较分析,认为β亚基氨基酸序列在红藻分类上具有重要意义.Cloning and sequencing of the gene encoding β subunit of phycoerythrin of a red alga-Corallina officinalis were reported firstly. The sequence contained 983 nucleotides, including 10 nucleotidcs of upstream before the initiation codon of β subunit, 534 nucleotides of β subunit （encoding 177 amino acids） , 101 nucleotides for gene spacer and 338 nucleotides of 5′-terminal of α subunit （encoding 112 amino acids）. Similarities in the nucleotide level and amino acid level with nine known red alga PE genes were analyzed. The important taxonomic value of β subunit was suggested in alga taxonomy.

关 键 词：珊瑚藻 藻红蛋白 Β亚基 脱辅基蛋白基因 基因克隆 序列分析 

分 类 号：Q949.293.3[生物学—植物学] Q785