硼中子俘获法诱导C6胶质瘤细胞凋亡  

Boron neutron capture inducing apoptosis in C6 glioma cells

作　　者：章翔[1] 曹锐峰[1] 江新标[2] 张剑宁[1] 费舟[1] 肖艳[2] 王西玲[1] 梁景文[1] 

机构地区：[1]中国人民解放军第四军医大学西京医院全军神经外科研究所,西安710032 [2]西北核技术研究所

出　　处：《中华放射肿瘤学杂志》2005年第5期451-455,共5页Chinese Journal of Radiation Oncology

基　　金：国家自然科学基金资助项目(10375047)

摘　　要：目的观察硼中子俘获法(BNCT)对体外培养C6细胞的作用并探讨其原理。方法实验分为未照射组(0 Gy)、γ射线组(48、Gy)、反应堆组(3 Gy)、BNCT组(48、Gy)。四甲基偶氮唑蓝(MTT)法测定照射后144 h内各组细胞生长曲线。光镜、荧光显微镜、电镜观察BNCT后细胞形态学改变,流式细胞术检测细胞凋亡率,成克隆分析法测定细胞存活分数。结果BNCT组细胞早期即出现凋亡的典型形态学改变。BNCT 48、Gy组细胞生长抑制作用显著强于等剂量的γ射线照射组(P<0.01)。BNCT 48、Gy组处理后48 h凋亡率分别为63.2%、88.3%,显著高于各组(P<0.01)。BNCT 48、Gy组存活分数均<0.0001,显著低于各组(P<0.01)。结论与γ射线照射相比较,BNCT对C6胶质瘤具有较高的相对生物学效应,其作用是通过诱导C6细胞凋亡而实现的。Objective To investigate the effect and mechanism of boron neutron capture therapy （BNCT） in C6 glioma cell line. Methods C6 cells in exponential phase were divided into 6 groups： untreated control, ^60Co γ4 Gy, ^60Co γ8 Gy, nuclear reactor exposure without boronophenylalanine（BPA） 3 Gy, BNCT 4 Gy and BNCT 8 Gy. Cellular morphological change was observed by an inverted microscope, light microscope, fluorescence microscope and electronic microscope. Flow cytometry was used to determine the percentage of apoptosis, necrosis and normal cells 48 h after irradiation. Colony forming assay was used to calculate cell surviving fraction. Results Typical morphological changes of apoptosis were observed early after irradiation in BNCT group, with a significant increase in apoptotic rates was observed 48 h after irradiation with 63.2% and 88.3% for BNCT 4 Gy and 8 Gy group, respectively（ P 〈 0.05 vs untreated control）. BNCT showed stronger growth inhibition than the same dose of γ-ray irradiation（ P 〈 0.01 ）. The surviving fraction of BNCT group was significantly lower than that of reactor and γ-ray control group（ P 〈 0.01）. Conclusions Through inducing more apoptosis, BNCT shows much higher relative biological effect than γ irradiation on C6 cell line.

关 键 词：硼中子俘获法 细胞系 肿瘤 细胞凋亡 瘤细胞凋亡 C6胶质瘤 俘获法 中子 细胞形态学改变 Γ射线照射 

分 类 号：R739.4[医药卫生—肿瘤]