代谢性谷氨酸受体1α在大鼠马桑内酯点燃致癫模型中的表达  

Expression of Metabotropic Glutamate Receptor 1α in Different Brain Areas of the Kindled Epilepsia Models of Rats by Coriaria Lactone

作　　者：林旭[1] 周东[1] 鄢波[1] 王琴[1] 张尚福[2] 

机构地区：[1]四川大学华西医院神经内科,成都610041 [2]四川大学华西医院病理科

出　　处：《四川大学学报（医学版）》2005年第5期649-652,共4页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号30370513)资助

摘　　要：目的探讨代谢性谷氨酸受体1α(MGLUR1α)在马桑内酯(CL)点燃致癫模型大鼠脑组织不同区域表达的差异.方法 35只雄性SD大鼠随机分为对照组(n＝5,大鼠予1.0 ml/kg生理盐水肌注)和3个不同剂量CL点燃致癫模型组(分别予0.75、1.0、1.25 ml/kg马桑内酯提取液肌注).分别对点燃、未点燃、对照组大鼠行皮层脑电图描记、取脑组织作HE和MGLUR1α的免疫组化染色,观察MGLUR1α在脑组织不同区域的表达.结果点燃组大鼠在肌注马桑内酯后产生了1～5级的痫性发作,与皮层脑电图的表现相一致.MGLUR1α在点燃组大鼠海马以及海马以外的颞叶皮质的表达较之未点燃和对照组增强(P<0.05),这种表达的增强在神经元与神经胶质细胞间未发现有明显的差异.结论 MGLUR1α在点燃大鼠海马和海马以外的颞叶皮质的表达较之未点燃和对照组增强,可能参与了癫痫的发生.Objective To detect the expression of Metabotropic Glutamate Receptor 1α（MGLUR1α）in the different brain areas of the kindled epilepsia models of rats by Coriaria Lactone（CL）. Methods Thirty male SD rats were divided into 3 groups and were given intramuscular injections of 0. 75, 1.0 and 1.25 ml/kg Coriaria Lactone respectively. 5 rats in the control group were given intramuscular injection of 1.0 ml/kg saline. After the kindling models were completed, the EcoG of all groups were recorded with the multi-electrophysiology recorder to view whether the EcoG of kindled rats accord with their seizures and to know whether there are differencees in EcoG among the kindled, non-kindled and control groups. The coronary sections of brain tissue were HE stained and MGLUR1α immunohistochemistry stained, and were observed under light microscope. Results The kindled rats had all grade （Simialowski scale）seizures after CL injection and their seizures accorded with their EcoG features. The MGLUR1α expression of kindled rats in hippocampus and temporal cortex outside hippocampus was stronger than that of non-kindled and control rats （P〈0. 05）; the strong expression was noted to be of no obvious difference between neuron and glia. Conclusion The MGLUR1α expression of kindled rats in hippocampus and temporal cortex outside hippocampus is stronger than that of non-kindled and control. It is possible that MGLUR1α particioates in epileptic seizures.

关 键 词：马桑内酯 点燃 动物模型 代谢性谷氨酸受体1α 

分 类 号：R742.1[医药卫生—神经病学与精神病学]