胰腺癌基质调节蛋白表达与肥大细胞计数的相关性研究  

Study on Relationships Between Expressions of Matrix Regulated Proteins and Count of Mast Cell in Pancreatic Cancer Tissues

作　　者：杨竹林[1] 李永国[1] 刘栋才[1] 邓星辉[1] 苗雄鹰[1] 钟德玝[1] 

机构地区：[1]中南大学湘雅二医院肝胆疾病研究室,长沙410011

出　　处：《中国普外基础与临床杂志》2005年第5期488-491,501,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的研究胰腺癌组织中细胞外基质金属蛋白酶诱导因子(EMMPRIN)、基质金属蛋白酶1(MMP1)、MMP9及金属蛋白酶组织抑制因子1(TIMP1)的表达和肥大细胞计数(MCC),并探讨其临床病理意义及其相关性。方法51例胰腺癌手术切除标本,经10%甲醛固定后常规制作石蜡包埋切片,行ABC免疫组化染色。结果胰腺癌组织中EMMPRIN、MMP1、MMP9和TIMP1表达阳性率分别为56.9%、54.9%、60.8%和49.0%,其评分值分别为(2.5±1.5)分、(2.3±1.9)分、(2.4±1.6)分和(1.9±1.6)分。高分化腺癌和无转移癌的前三项指标的阳性率及其评分值明显低于低分化腺癌和转移癌,中分化腺癌(除MCC及MMP9评分值外)上述指标亦明显低于低分化癌,而TIMP1则相反,其差异均有统计学意义(P<0.05,P<0.01)。胰腺癌组织中MCC平均值为(16.1±6.8)个/HP,高分化腺癌和无转移癌的MCC明显低于低分化腺癌和转移癌(P<0.01)。EMMPRIN、MMP1和MMP9阳性病例及TIMP1阴性病例的MCC明显高于EMMPRIN、MMP1、MMP9阴性病例及TIMP1阳性病例(P<0.01)。MCC与EMMPRIN、MMP1及MMP9评分值呈正相关,与TIMP1评分值呈负相关;EMMPRIN、MMP1和MMP9评分值之间均呈正相关,但与TIMP1评分值之间均呈负相关。结论MCC和EMMPRIN、MMP1、MMP9及TIMP1的表达均是反映胰腺癌进展和预后的重要标记物,它们可能相互影响和共同调节胰腺癌侵袭和转移发生的潜力。Objective To investigate the expression of extracellular matrix metalloproteinase inducer（EMMPRIN）, matrix metalloprotelnase-1 （MMP1）, MMP9, tissue inhibitors of metalloproteinase-1 （TIMP1） and the mast cell count （MCC） and to detect their clinicopathologic significance and relationship in pancreatic cancer tissues. Methods Immunohistochemical method of avidin-biotin complex was used for those 5 targets on the routinely paraffin-embedded sections of surgical resected specimen of 51 cases with pancreatic carcinoma. Results The positive rates of EMMPRIN,MMP1 ,MMP9 and TIMP1 were 56.9% ,54.9% ,60.8% and 49.0% and its scoring were 2.5±1.5,2.3±1.9,2.4±1.6 and 1.9±1.6 respectively. The mean of MCC was （16.1±6.8）/HP in total cases. The positive rates or scorings of EMMPRIN, MMP1, MMP9 and MCC were significantly lower in high differentiated or without-metastatic cases than in low differentiated or with-metastatic ones（P〈0.05 or P〈0.01）, and those targets （except MCC and scoring of MMP9 ） of middle differentiated ones were lower than those of low differentiated while that of TIMP1 was opposite（P〈0. 01）. The MCC showed significantly higher in the positive cases of EMMPRIN, MMP9 and MMP9 or negative cases of TIMP1 than in the negative ones of EMMPRIN, MMP1 and MMP9 or positive ones of TIMP1. The closely positive correlations were found among the MCC and the scoring of EMMPRIN, MMP1 and MMP9. The closely negative correlations existed among the scoring of TIMP1 and the other four targets. Conclusion The MCC and the expressions of EMMPRIN, MMP1, MMP9 and TIMP1 might be important biological markers for reflecting the progression and the prognosis of pancreatic carcinoma. They might have co-regulated effects on the potentials of invasion and metastasis of pancreatic carcinoma or other malignant lesions.

关 键 词：胰腺肿瘤 细胞外基质金属蛋白酶诱导因子 基质金属蛋白酶类 金属蛋白酶组织抑 制因子 肥大细胞 

分 类 号：R735.9[医药卫生—肿瘤]