AD大鼠模型的MRI及MR体积测量与病理对照研究  

Correlation of MRI and MR Volumetric Analysis with Pathology in Animal Model of Alzhiemer′s Disease

作　　者：宋焱[1] 祁吉[1] 尹建忠[1] 

机构地区：[1]天津医科大学第一中心医院放射科

出　　处：《放射学实践》2005年第9期814-817,共4页Radiologic Practice

基　　金：天津市自然科学基金资助项目(013615911)

摘　　要：目的:探讨早期AD大鼠模型的影像学改变及其病理学基础.方法:64只大鼠随机分为实验组和对照组,每组各32只.实验组和对照组分别于海马区注射Aβ(1-40)和生理盐水.于注射后1周、2周、4周、6周、8周,进行AD大鼠头颅MRI检查、海马MR体积测量及病理学检查(HE染色,刚果红染色,银染,电镜).结果:病理证实实验组AD大鼠模型成功建立.模型建立6～8周,神经病理学检查可见胶质细胞增生、不典型老年斑和神经纤维缠结,无神经元缺失;AD大鼠海马MR体积测量未发现海马萎缩,但MRI可见注药侧颞极部蛛网膜下腔增宽.结论:Aβ(1-40)海马注射大鼠模型是研究AD影像学改变及其病理基础的一种有用的动物模型.MRI可于早期提示AD以便临床进行进一步检查.Objective：To investigate the MRI findings in animal model of early stage of AD and its pathologic mechanism. Methods： 64 mice which were equally divided into experimental group [（induced by A^β （1-40） injection into hippocampus）] and contrast group （injection of normal saline into hippocampus） . These two groups of animal model were divided randomly into 5 sub-groups with 6 mice in each subgroup. Examinations including MRI, MR volumetric analysis of hippocampus and neural pathologic examination （including HE,Congo red and Bielschowsky pigmentation） were undertaken on these subgroups at different time point;l,2,4,6 and 8 week after injection. Another 4 mice underwent electron microscope examination. Results;Successfully setup of AD model of mouse were proved by neural pathology examination. At 6～8 weeks after setup of model,gliosis,atypical aging speckle and twinning of neural fibers were present and can be revealed by neural pathology,but without concomitance of absence of neuron. MRI show enlargement of subarachnoid space surrrounding temporal pole,while MR volumetric analysis did not show atrophy of hippocampus. Conclusion; The mouse model induced by injection of A^β （1-40） into hippocampus is a preferable model in the investigation of imaging manifestations and their pathology mechanism of AD. Existence of early stage of AD can be suggested by MRI and further clinical examination could be undertaken.

关 键 词：磁共振成像 阿尔茨海默病 动物模型 

分 类 号：R749.1[医药卫生—神经病学与精神病学]