广西肝癌高发区氧化性DNA损伤与修复酶hOGG_1的表达和多态性关系的研究  

Detection of oxidative DNA damage,hOGG_1 expression and polymorphisms in hepatocellular carcinoma at endemic regions in Guangxi

作　　者：刘志明 [1] 彭涛 [1] 严律南 [2] 彭民浩 [1] 黎乐群 [1] 李家荃 [1] 梁任祥  韦忠亮  Shen Hanming  Ong Choon Nam  

机构地区：[1]530021,南宁市,广西医科大学附属第一医院普通外科 [2]610041,成都市,四川大学华西医院普通外科 [3]广西扶绥县肿瘤防治研究所 [4]新加坡国立大学社区、职业与家庭医学部

出　　处：《中华肝胆外科杂志》2005年第8期505-509,共5页Chinese Journal of Hepatobiliary Surgery

基　　金：广西自然科学基金(桂科自99120280236030);广西卫生厅医药卫生科研基金(Z2001087);SingaporeSciencGrant;No.R-186-000-044-213资助

摘　　要：目的研究广西肝癌高发区未成年人的氧化性DNA损伤情况。方法采用流式细胞技术检测外周血白细胞DNA7,8-dihydro-8-oxoGuanine(8-oxoG)及其修复酶hOGG1的水平,采用PCR-SSCP技术检测hOGG1-Cys326Ser多态性。结果8-oxoG与hOGG1表达水平高度相关(0·48<r<0·88,P<0·001)。高发区肝癌户未成年人(n=21)与对照(n=63)相比,hOGG1表达水平较高(P<0·01)但8-oxoG水平较低(P<0·05)。城市对照被动吸烟者hOGG1表达水平较高(P<0·05)。8-oxoG和hOGG1水平与体重指数负相关;hOGG1与年龄正相关。在12岁附近有一8-oxoG水平峰。hOGG1326Ser携带者较hOGG1326Cys携带者8-oxoG水平高。结论人群模型的氧化性DNA损伤研究,可从新的角度为了解环境-基因相互作用提供资料。Objective To study the oxidative DNA damage in adolescents of hepatocellular carcinoma （HCC） families in Guangxi, China. Methods Peripheral leukocyte DNA, 7, 8-dihydro-8-ox-oGuanine （8-oxoG） and repair enzyme hOGG1 were quantified by flow cytometry, hOGG1Ser^326 Cys polymorphism was determined by PCR-SSCP. Results There was a positive correlation between 8-oxoG and repair enzyme hOGG1 expression （0. 48〈 r〈 0. 88, P〈0. 001）. Children of HCC families （n= 21） in Fusui county had significantly higher level of hOGG1 （P〈0. 01 but lower level of 8-oxoG （P〈0.05） than that in the controls （n= 63） in Nanning city. Nanning children exposed to passive smoking had higher hOGG1 expression （P〈0. 05） than that in those not exposed to passive smoking. 8-oxoG and hOGG1 were negatively correlated with body mass index while hOGG1 was positively correlated to the age. There was a peak of 8-oxoG level around the age of 12 years old. Carriers of hOGG1^326Ser genotype had higher level of 8-oxoG than those of hOGG1^326 Cys. Conehtsions The study of oxidative DNA damage in population provides a new insight for gene-environment interaction.

关 键 词：癌 肝细胞 损伤 DNA 8-oxoG HOGG1 氧化性DNA损伤 肝癌高发区 修复酶 多态性 广西 

分 类 号：R735.7[医药卫生—肿瘤]