氟尿嘧啶区域动脉灌注对重症急性胰腺炎大鼠细胞凋亡的影响  

The Effect of Regional Intra-arterial Infusion with Fluorouracil on Apoptosis in Rats with Severe Acute Pancreatitis

作　　者：高友兵[1] 周涛[1] 汪训实[1] 卢绮萍[1] 

机构地区：[1]广州军区武汉总医院普通外科,湖北武汉430070

出　　处：《医学临床研究》2005年第9期1266-1267,1270,共3页Journal of Clinical Research

摘　　要：【目的】探讨氟尿嘧啶区域动脉灌注对重症急性胰腺炎(SAP)大鼠细胞凋亡的影响。【方法】SD大鼠64只,随机分为假手术组(A组),SAP组(B组),氟尿嘧啶外周静脉注射组(C组),氟尿嘧啶区域动脉灌注组(D组)。测定各组大鼠的血清淀粉酶(Amy),观察胰腺病理变化,并检测胰腺腺泡细胞的凋亡率。【结果】①制成SAP模型后,各组Amy水平显著上升,氟尿嘧啶治疗后可使Amy下降,其中氟尿嘧啶区域动脉灌注组下降更为明显。②氟尿嘧啶治疗可使胰腺腺泡细胞凋亡率明显上升,胰腺病理损害程度减轻,氟尿嘧啶区域动脉灌注者作用更强。【结论】①SAP时,应用氟尿嘧啶可诱导胰腺细胞凋亡,减轻胰腺病理损害,是治疗SAP的方法之一。②区域动脉灌注是治疗SAP的增效途径。[Objective]To study the effects of regional intra-arterial infusion with fluorouracil （Fu） on apoptosis in rats with severe acute pancreatitis （SAP）. [Methods]SD rats were divided into four groups randomly： Sham-operative group （group A）, SAP group （group B）, group of peripheral vessel infusion of Fu （group C）, group of regional intra-arterial perfusion of Fu （groupD）. The contents of serum amylase and the pancreatic histologic changes were observed and apoptosis of pancreatic acinar cells was detected by triphosphate-biotin nick-end Labelling （TUNEL）. [Results]①The serum amylase level of each group after establishment of model was much higher than before, treatment with Fu decreased the serum amylase level, regional intra-arterial perfusion of Fu had the most remarkable effectiveness. ②Treated with Fu, the apoptotic ratio of acinar cells improved, the pathological damage severity of pancreas was significantly attenuated, especially in group D.[Conclusion]①During the course of SAP, treatment with Fu could improve the apoptotic ratio of acinar cells, has beneficial effect on pancreas damage.②Regional intra-arterial perfusion is one of the means of improving therapeutic effect of drugs against SAP.

关 键 词：胰腺炎/药物疗法 急性病 大鼠 脱噬作用 灌注 局部 氟尿嘧啶 

分 类 号：R576[医药卫生—消化系统]