不同工艺六味地黄丸制剂对小鼠溶血素含量的影响  

Effect of Liuwei Dihuang Pills Prepared by different Kinds of Techniques on Hemolysin Content in Mice

作　　者：张辉[1] 龙致贤[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中药新药与临床药理》2005年第5期339-342,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的观察六味地黄丸四种不同工艺对小鼠溶血素抗体生成的影响,比较各种工艺的药效差异,以此考察工艺。方法以绵羊红细胞(SRBC)为免疫原,分别用地塞米松和环磷酰胺建立免疫低下模型,给小鼠灌服六味地黄丸各工艺制备的溶液后,测定小鼠的溶血素含量,比较各工艺制剂疗效的差异性。结果环磷酰胺模型组与各给药组对SRBC致敏小鼠的溶血素生成反应低下,半数溶血值(HC50)整体偏低,工艺Ⅱ、Ⅲ、Ⅳ组其均值低下,与模型组比较有显著性差异。地塞米松模型组与工艺Ⅰ各剂量组、浓缩六味地黄丸组比较差异不显著,且工艺Ⅰ高中低剂量组间差异无统计学意义。结论六味地黄丸服用量存在减小可能,但仍需改进制剂工艺并进一步验证;本方主治肾阴虚证,在选择免疫低下造模药时,建议选地塞米松为宜;实验时需增加其他观察指标以全面反映其疗效,反映剂型改革的合理性。Objective To compare the pharmacodynamic actions of Liuwei Dihuong Pills （LDP） prepared by four kinds of techniques on hemolysin contents in mice. Methods Sensitized by SRBC, immuno-inhibited mice models were induced by dexamethasone and cyclophosphane respectively. The hemolysin contents in the mice fed with LDP prepared by four kinds of techniques were observed to compare the pharmacodynamic actions. Results In experiment Ⅰ that immuno-inhibited mice models were induced by cyclophosphane, the half hemolysin concentration （HC50） was low in the model group and LDP groups, especially in the LDP groups of technique 1], I~ and 1V. In experiment 2 that immuno-inhibited mice models were induced by dexamethasone, the difference of HC50 was insignificant between the blank control group, LDP groups of technique Ⅰ and concentrated LDP group, the difference also being not significant in LDP groups of technique I at different dosages. Conclusion The dose of LDP can be reduced according to tire experiment, however, the process technique of LDP needs to be improved. For this prescription is indicated for the treatment of yin-deficiency syndrome, dexamethasone can be used as the proposed drug for the establishment of the imumno-inhibited models. To confirm the reasonability of formula revolution, more parameters should be added to observe the pharmacodynamic actions.

关 键 词：六味地黄丸 不同工艺 溶血素 

分 类 号：R285.5[医药卫生—中药学]