巨型辐花海葵荧光蛋白类似物基因的原核表达比较  

Comparative Analysis of Recombinant Expression of GFP Homolog from Heteractis Magnifica in E.Coli

作　　者：涂洪斌[1] 朱俊杰[2] 杨文利[2] 姜孝玉[2] 陈慧萍[2] 徐安龙[2] 

机构地区：[1]广州医学院实验医学研究中心肿瘤研究所,广东广州510182 [2]中山大学生命科学学院生物化学系海洋生物功能基因组开放实验室,广东广州510275

出　　处：《广州医学院学报》2005年第3期1-6,共6页Academic Journal of Guangzhou Medical College

基　　金：广州医学院博士启动基金(025105)

摘　　要：目的:探讨巨型辐花海葵荧光蛋白类似物基因hmGFP的生物学特性及应用的可能性。方法:构建了hmGFP的两个原核表达载体并进行诱导表达。结果:低温下hmGFP获得了功能性表达而发光。深入比较了重组巨型辐花海葵荧光蛋白类似物基因hmGFP在两个表达体系中的表达差异,其中pETTRX-hmGFP的重组蛋白表达水平和可溶性均比pET21b-hmGFP高。结论:对pETTRX-hmGFP的表达条件进行了优化,为下一步hmGFP成熟重组蛋白的纯化及其荧光波谱分析奠定了良好基础。Objective：To gain insight of biological characteristics and application feasibility for GFP homolog hmGFP from Heteractis magnifica. Methods： Two different prokaryotie expression systems （ pET21 b and pETTRX） of hmGFP were constructed and recombinant hmGFP can be both functionally expressed under low temperature. The expression difference between pET21b-hmGFP and pETTRX-hmGFP were further investigated. Results：The expression level and the solubility of the recombinant hmGFP protein in pETTRX were better than that in pET21b. Conclusion： The optimal pETTRX-hmGFP expression condition was established for next step of purifying mature recombinant hmGFP protein to characterize its fluorescent spectrum.

关 键 词：巨型辐花海葵 荧光蛋白类似物基因 原核表达 

分 类 号：Q786[生物学—分子生物学]