透明质酸酶和油酸增强外源基因在机体内的表达  

Enhancement of the Expression of Exogenous Gene in Vivo by Oleic Acid and Hyaluronidase

作　　者：任智慧[1] 朱开春[1] 金华利[2] 江睿[2] 张富春[1] 王宾[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐830046 [2]中国农业大学生物学院农业生物技术国家重点实验室,北京100094

出　　处：《中国生物工程杂志》2005年第9期59-63,共5页China Biotechnology

基　　金：国家"863"计划(2003AA241110和2004AA213102);国家自然科学基金资助项目(30271220)

摘　　要：以研究外源基因有效发送至靶细胞并高效表达为目的,为了获得能提高外源基因在机体内表达量的增强剂,以pCMVβ-gal作为报告基因,分别辅以油酸和透明质酸酶两种不同的生化试剂,注射小鼠后肢腿部肌肉。通过检测在两种不同的生化试剂作用下,pCMVβ-gal在机体内表达量的差异,以期获得能提高外源基因表达量的增强剂。结果证明油酸和透明质酸酶均能有效提高pCMVβ-gal在小鼠体内的表达量,其中透明质酸酶引起的增强效果更为显著,是较好的基因表达增强剂。To achieve a higher efficiency with the exogenous gene expression is still a big challenge for the non-viral vector delivery approach in despite of the technology been improved over a decade. The main obstacle is to delivery the exogenous gene into tissues in vivo with a mimmal loss ann with a maximal penetration into cell membrane. Several strategies have been applied to achieve this goal previously, such as lipidsome or polymer encapsulations, gene gun, in situ electroporation. Chemicals with a permeable property might be considered as facilitators to enhance the egiciency of the exogenous gene entry. Owing to the easiness in application and cost effectiveness, two biochemical reagents have been incorporated to facilitate such delivery that may improve the exogenous gene expression in vivo. After injections of the mice intramuscularly with a pCMVβ-gal construct mixed with the oleic acid or hyaluronidase, it showed that the expression of the β-gal gene was enhanced by either of the facilitators compared with the mice injected with the β-gal gene formulated in saline. The enhanced expressions are apparently associated with the characteristics of the chemicals used since they are enable to increase the permealibility of ceils as previous documented. An evaluation of DNA vaccine formulated in the two facilitators is currently underway. Preliminary observations on the enhancement of the expressed exogenous gene using the two biochemical reagents may lead to the improvement for the development of DNA vaccination and gene therapy.

关 键 词：pCMVβ-gal 体内表达 油酸 透明质酸酶 表达增强剂 外源基因 体内 生化试剂 表达量 增强剂 

分 类 号：Q78[生物学—分子生物学] TS202[轻工技术与工程—食品科学]