5氮杂胞苷对HT29细胞DLC-1基因表达和细胞增殖活性的影响  

Effect of 5-Aza-CdR on DLC-1 expression and proliferation of human colorectal cancer cell line HT29

作　　者：伍健[1] 金月玲[1] 黄培林[1] 

机构地区：[1]东南大学基础医学院病理学系,江苏南京210009

出　　处：《现代医学》2005年第3期141-144,共4页Modern Medical Journal

基　　金：国家自然科学基金资助项目(30471937)。

摘　　要：目的探讨去甲基化药物5氮杂胞苷对肠癌细胞系HT29的DLC1基因表达和细胞增殖活性的影响。方法使用浓度为5μmol·L-1和10μmol·L-1的去甲基化药物5氮杂胞苷处理HT29细胞,通过MTT实验观察细胞增殖活性的差异,RTPCR技术检测DLC1mRNA表达强度的变化。结果经药物处理后,HT29细胞的增殖活性受到抑制,DLC1mRNA的表达明显增强。结论HT29细胞DLC1mRNA的表达下调可能是由于启动子区域高甲基化所致,DLC1基因具有抑制肿瘤细胞生长的功能,其可能参与了结肠癌的发病机制。Objective To explore the effect of 5-Aza-CdR on gene DLC-1 expression and cell proliferation in human colorectal cancer cell line H329. Methods The colorectal cancer cell line H329 was treated with 5 μmol·L^-1 and 10 μmol·L^-1 5-Aza-CdR. The activation difference in cell growth was observed by MTr assay, and the changes of the DLC-1 mRNA expression were detected by RT-PCR. Results Compared with the control, the cell growth was reduced after treatment, and the expression of the DLC-1 mRNA was increased. Conclusion The expression of DLC-1 mRNA in the cell line H329 is downregulated resulting from the hypennethylation of CpG islands within the promoter regions, and DLC-1 plays an inhibitive role on cancer cell growth, which is possibly involved in the colorectal cancer pathogenesis.

关 键 词：肠癌细胞系 DIE-1基因 5氮杂胞苷 去甲基化 细胞增殖 

分 类 号：R730.51[医药卫生—肿瘤]