喉癌组织中Survivin和p63的表达及临床意义  

Expression and biological significance of Survivin and p63 in laryngeal squamous cell carcinoma

作　　者：温晓霞[1] 高英[1] 季文樾[2] 

机构地区：[1]中国医科大学附属第四医院耳鼻咽喉科,沈阳110032 [2]中国医科大学附属第一医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉科杂志》2005年第18期833-835,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的:探讨Survivin、p63在喉鳞状细胞癌(LSCC)组织中的表达及与LSCC发生、发展的关系和生物学意义。方法:应用原位分子杂交方法,检测SurvivinmRNA、p63mRNA在47例LSCC组织、癌旁组织及14例正常喉组织中的表达。结果:①SurvivinmRNA在正常喉组织中不表达;在LSCC组织、癌旁组织中的阳性表达率分别为59.6%(28/47)、25.5%(12/47),其差异有统计学意义(P<0.05);组织分化程度越低、TNM分期越晚、淋巴结转移率越高,则SurvivinmRNA表达越强(均P<0.05)。②p63mRNA在LSCC组织、癌旁组织中的阳性表达率分别为91.5%(43/47)、93.6%(44/47),分别与正常喉组织(57.1%)比较,差异有统计学意义(P<0.05);p63mRNA与喉癌临床病理特征无关。结论:Survivin在LSCC中表达上调,提示其通过抑制细胞凋亡在LSCC的发生、发展过程中起重要作用,可能成为LSCC基因治疗的新靶点;其高表达预示肿瘤有较高的侵袭性和不良预后。p63对LSCC生物学行为的影响较小。Objective：To investigate the expression of Survivin and p63 and their biological significance in laryngeal squamous cell carcinomas（LSCC）. Method： The expression of Survivin and p63 in 47 cases of tumor tissues,adjacent and normal tissues were detected using in situ hybridization. Result： Percentage of Survivin expres sion was 59.6% （28/47）, 25.5% （12/47） in tumor tissues and adjacent tissues respectively. In contrast, normal laryngeal tissues did not express Survivin. Over expression of Survivin was strongly associated with tumor grade, TNM stage and cervical nodal metastasis. Percentage of p63 expression was 91. 5% （43/47）, 93. 6% （44/47）, 57.1% （8/14）in tumor tissues,adjacent and normal tissues respectively. The expression of p63 was not related to any clinicopathological features. Conclusion：Survivin may play an important role in the pathway of carcinogenesis and progression of LSCC and it may be identified as a new therapeutic target. The up-regulation of the expression of Survivin predicts more invasion and poor prognosis, p63 has less effect on biological behaviors of LSCC.

关 键 词：喉肿瘤 癌 鳞状细胞 基因 p63 基因 Survivin 

分 类 号：R739.65[医药卫生—肿瘤]