克拉霉素与紫色素的荷移反应及其测定  

Spectrophotometric Determination of Clarithromycin Based on the Charge Transfer Reaction between Clarithromycin and Purpurin

作　　者：李华侃[1] 肖井坤[2] 

机构地区：[1]锦州医学院,锦州121001 [2]大连海事大学环境科学与工程学院,大连116026

出　　处：《分析化学》2005年第9期1327-1329,共3页Chinese Journal of Analytical Chemistry

摘　　要：建立了一种测定克拉霉素的荷移分光光度法.克拉霉素与紫色素在乙醇溶液中发生电荷转移反应,荷移络合物在548nm处有最大吸收,表观摩尔吸光系数是4.49×103L*mol-1*cm-1, 该络合物的组成是1:1; 稳定常数是3.48×104;药物浓度在10～150 mg/L 范围内服从比耳定律.当克拉霉素浓度为100 mg/L时,6次测定结果的相对标准偏差为1.2%.利用本法测定了克拉霉素制剂中有效成分的含量,并与文献法进行比较,二者结果基本吻合,回收率在97.0%以上.A spectrophotometric method for the determination of clarithromycin has been developed based on the charge transfer reaction between clarithromycin as donor and purpurin as acceptor in alchol medium. The apparent molar absorptivity of complex is 4.49×10^3 L·mol^-1·cm^-1 at 548 nm, while the composition ratio and stability constant of charge transfer complex are 1 ： 1 and 3.48×10^4 respectively. Beer＇s law is strictly obeyed in the range of 10 - 150 mg/L of clarithromycin. The average recovery is over 97.0% . The relative standard deviation of six determinations is 1.2% when the concentration of clarithromycin is 100 mg/L. The method has been applied to the analysis of some clarithromycin samples with satisfactory results. Compared with other common method, spectrophotometric determination is a more convenient method due to its cheap equipment and rapid measurement.

关 键 词：电荷转移反应 紫色素 克拉霉素 分光光度法 测定结果 荷移反应 表观摩尔吸光系数 荷移络合物 相对标准偏差 药物浓度 

分 类 号：TQ460.7[化学工程—制药化工]