趋化因子在实验性自身免疫性神经炎中的动态表达  

Dynamic Expression of Chemokines in Experimental Autoimmune Neuritis

作　　者：周文斌[1] 周颖[1] 肖波[1] 

机构地区：[1]中南大学湘雅医院神经内科,湖南长沙410008

出　　处：《中国神经免疫学和神经病学杂志》2005年第5期262-264,i0002,共4页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的探讨趋化因子在实验性自身免疫性神经炎(EAN)中的作用。方法用兔坐骨神经匀浆免疫Wist-ar大鼠,观察免疫后大鼠的发病情况和病理改变,通过免疫组化测定趋化因子在坐骨神经中的动态表达。结果EAN大鼠于免疫后第9天开始出现症状,第15天症状达高峰,病理表现为炎性细胞浸润和脱髓鞘。趋化因子MCP-1表达在第9天达高峰,随后逐渐下降,前后时相点相比差异均有显著性(P<0.05),且与对照组相比差异也有显著性(P<0.001)。趋化因子MIP-1α和RANTES的表达有着相似的动态变化,在第15天疾病高峰期表达最高,随后逐渐下降,且与对照组相比差异有显著性(P<0.001)。结论趋化因子MCP-1在EAN发病早期可能起一定作用,MIP-1α和RANTES可能与EAN的病情进展有关。Objective To explore the role of chemokines in experimental autoimmune neuritis（EAN）. MethodsEAN were induced in Wistar rats by immunization with rabbit aciatic nerves homogenate and complete Freund adjuvant（CFA）. We observed the clinical signs of rats and pathological changes in the sciatic nerves of rats. Dynamics of production of MCP-1, MIP-1α, RANTES in the sciatic nerves of experimental rats were detected by immunohistochemistry technology. Results Rats developed EAN with maximum of clinical signs on day 15 after immunized, which was characterized by inflammatory cell infiltration and demyelination in the sciatic nerves. The maximum of MCP-1 positive cells in the sciatic nerves were detected on day 9. However, peak numbers of MIP-1α and RANTES positive cells were detected on day 15 ,correlating with development of severe clinical signs. Conclusions Chemokine MCP-1 might play an initiatory role in the course of EAN. The expression of MIP-1α and RANTES might correlate with the process of disease.

关 键 词：实验性自身免疫性神经炎 趋化因子 

分 类 号：R745.43[医药卫生—神经病学与精神病学]