180例无精子症或严重少精子症不育男性的Y染色体微缺失检测  

Detection of Y chromosome microdeletions in 180 infertile men with azoospermia or severe oligozoospermia

作　　者：方小武[1] 谭志伟[1] 雷继敏[1] 韦剑洪[1] 梁少霞[1] 

机构地区：[1]中山市博爱医院生殖遗传医学中心,广东528403

出　　处：《中国优生与遗传杂志》2005年第9期30-31,共2页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨不育男性无精子症或严重少精子症与Y染色体微缺失之间的关系。方法利用9个Y染色体特异序列标签位点,以多重PCR法检测无精子症或严重少精子症患者的Y染色体微缺失情况。结果180例无精子症或严重少精子症患者中共检出Y染色体微缺失15例,缺失率为8.3%。精液正常者(对照组)20例未发现Y染色体微缺失。9例Y染色体微缺失的无精子症患者睾丸细胞学检查均未发现精子。结论Y染色体微缺失是造成男性精子发生障碍的常见病因之一。Objective：to assess the relationship between azoospermia or severe oligozoospermia and Y chromosomal micredeletions. Methods ： Infertile men with azoopermia or severe oligozoospermia were screened by multi - polymerese chain reactions with 9 Y chromosome specific sequence - tagged site labels. Results： Micredeletions were detected in 8.3 % （15/180） infertile men with azoospermia or severe oligozoospermia and in 0 of normospermic men. Among 9 azoospermic men with Y chromosome micredeletion, no sperm was found in testicular cell smears, Conclusion：Y chromosome micredelefion is one of the causes which result in male spermatogenetic obstacle.

关 键 词：男性不育 Y染色体微缺失 无精子症 严重少精子症 

分 类 号：R698.2[医药卫生—泌尿科学]