云南战区部队首次检出携带耶尔森菌HPI毒力岛基因irp^(-2)的大肠杆菌  

First detection of pathogenicity islands gene irp^(-2) of Yersinia enterocolitica HPI from Escherichia coli strains in the troops of Yunnan military region

作　　者：李刚山[1] 朱姝媛[1] 范泉水[1] 侯敏[2] 徐庆[2] 邱薇[1] 龙沛然[1] 周卫国[1] 尹惠琼[1] 

机构地区：[1]成都军区疾病预防控制中心军事医学研究所,昆明650032 [2]昆明市疾病预防控制中心,昆明650034

出　　处：《中国卫生检验杂志》2005年第10期1179-1180,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解携带小肠结肠炎耶尔森菌HPI毒力岛基因irp-2的大肠杆菌在云南战区部队腹泻患者粪便标本中的存在情况,并对其菌株做毒力试验。方法:用PCR扩增法检测毒力岛基因irp-2,小白鼠腹腔注射检测毒力。结果:从268份腹泻病人粪便中分离的大肠杆菌菌株中,检测出携带小肠结肠炎耶尔森菌HPI毒力岛基因irp-2的18株,检出率为6.72%(18/268)。毒力试验使小白鼠发病并死亡。结论:云南战区存在携带小肠结肠炎耶尔森菌HPI毒力岛基因irp-2的大肠杆菌,该菌对部队指战员的健康具有潜在性的威胁。Objective：To detect the occurrence and virulence of E. coli strains carrying pathogenicity islands gene irp^-2 of Yersinia enterocolitica HPI with night soil samples from diarrhoeal patients of the troops of Yunnan military region. Methods：Pathogenicity islands gene irp^-2 was detected by PCR amplification, and the virulence of E. coli strains was determined with mice by intraperitoneal injection. Results：18 E. coli strains carrying pathogenicity island gene irp^-2 of Yersinia enterocolitica HPI were detected from 268 night soil samples, representing a 6. 72% detection rate （ 18/268 ）, which further resulted in the occurrence of serious illness and death for experimental mice after intraperitoneal injection in virulence detection. Conelusion：E. coli strains carrying pathogenicity islands gene irp^-2 of Yersinia enterocolitica HPI occurred in the troops of Yunnan military region, which will bring putative threats to the health of commanders and fighters.

关 键 词：腹泻 毒力岛基因irp^-2 小肠结肠炎耶尔森菌 

分 类 号：R378.21[医药卫生—病原生物学]