大肠杆菌O157特异基因的PCR检测方法  

Detection of specific gene of E.coli O157 by PCR

作　　者：郑桂丽[1] 廖绍安[2] 李钏华[1] 龚玉娇[1] 莫自耀[1] 

机构地区：[1]广州市疾病预防控制中心,广州510080 [2]华南师范大学生命科学学院,广州510631

出　　处：《中国卫生检验杂志》2005年第10期1183-1185,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立一种灵敏、特异的检测肠出血性大肠杆菌O157的PCR方法。方法:针对大肠杆菌O157特异性基因rfbO157设计引物,扩增一497 bp的O157标识序列。结果:应用该PCR反应体系,对4株O157菌株和14株非O157菌株扩增结果表明,O157菌株均扩增出预期的497 bp片段,14株非O157菌株则为阴性。纯菌液检测灵敏度为60 cfu/PCR反应,模拟混合菌液检测灵敏度为400 cfu/PCR反应。结论:该方法用于肠出血性大肠杆菌O157的检测鉴定简便、快捷,具有很高的特异性和灵敏度,为O157的临床辅助诊断提供了有力手段。Objective：To develop a specific and sensitive PCR method for the detection of E. coli O157. Methods：The PCR assay was used to amplify a 497 bp product based upon primers derived from E. coli O157 highly specific region. Results：A fragment of 497 bp product was amplified from 4 O157 strains tested. No product was got from closely related bacteria. Conclusion： The PCR assay is rapid, specific and sensitive for the detection of E. coli O157, and useful for clinical diagnosis.

关 键 词：大肠杆菌O157 PCR rfbO157 

分 类 号：R378.21[医药卫生—病原生物学]