非小细胞肺癌p14^(ARF) p16^(INK4a)蛋白共表达及其临床意义  

The Co-expression and Clinical Significance of p14^(ARF) and p16^(INK4a) Proteins in Non-small Cell Lung Cancer

作　　者：李军果[1] 胡义德[1] 叶明福[2] 谢启超[1] 高丽莉[1] 孙玉兰[1] 杨永峰[1] 钱海洪[1] 

机构地区：[1]第三军医大学第二附属新桥医院全军肿瘤中心,重庆市400037 [2]第三军医大学第二附属新桥医院病理科,重庆市400037

出　　处：《中国肿瘤临床》2005年第18期1021-1024,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金资助(编号:30170293)

摘　　要：目的:探讨p14ARF、p16INK4a蛋白在非小细胞肺癌(NSCLC)组织中的表达、意义及相关关系。方法:采用免疫组织化学SP方法对103例NSCLC组织中p14ARF和p16INK4a蛋白的表达进行检测。结果:103例NSCLC组织中p14ARF、p16INK4a蛋白表达阴性率分别为70.87%和43.69%,差异有显著性(P<0.01)。其中35例p14ARF、p16INK4a蛋白表达共阴性,共阴性率达33.98%(35/103),鳞癌中共阴性率明显高于其它组织类型(P<0.01)。p14ARF、p16INK4a蛋白表达阴性相互间无显著相关性(P>0.05)。临床Ⅲ+Ⅳ期病例两种蛋白表达阴性率明显高于临床Ⅰ+Ⅱ期(P<0.05)。结论:NSCLC组织p14ARF、p16INK4a蛋白表达共阴性具有明显的组织学类型特异性,两种蛋白阴性表达是各自独立的事件。Objective： To study the expression and clinical significance of p14^ARF and p16^INK4a proteins and to explore the eorrelationship between negative expressions of p14^ARF and p16^INK4a proteins in non-small cell lung cancer （NSCLC）. Methods： The expressions of p14^ARF and p16^INK4a proteins were detected in 103 NSCLC samples by immunohistochemistry （S-P method）. Results： The negative expression of p14^ARF was detected in 73 （70.87%） of the 103 NSCLC cases. The other 30 cases showed positive immunohistochemical result. While the negative expression of pl61NK4＂was detected in 45 （43.69%） of 103 NSCLC cases. The other 58 cases showed positive immunohistochemical result. There was a significant difference between the negative rate of p1^ARF expression and of p16^INK4a expressions （P〈0.01）. In 103 NSCLC, 35 cases （33.98%） showed simultaneous loss in both proteins. The negative rates of p14^ARF and p16^INK4a co-expressions in squamous cell carcinoma, adenocarcinoma and bronchioloalveolar carcinoma were 55.88% （19/34）, 27.27% （9/33） and 19.44% （7/36）, respectively, and the squarnous cell carcinoma group was significantly higher compared to the others （P〈0.01）. Rank correlation analysis revealed that there was no correlation between negative expression of p14ARv and of p16^INK4a proteins in NSCLC （P〉0.05）. Of the 65 samples analyzed from NSCLC patients, the negative rate of p14^ARF and p16^INK4a expressions were 54.05%（20/37） and 35.14%（13/37） in the groups of clinical stage Ⅰ ＋Ⅱ respectively, the negative rate of p14^ARF and p16^INK4a expressions were 78.57%（22/28） and 60.71%（17/28） in the groups of clinical stage Ⅲ ＋ Ⅳ respectively. Either p14^ARF and p16^INK4a negative expression was closely related to clinical stage （P〈0.05）. Conclusion： Expression alteration of INK4a-ARF gene has the tendency of histopathological specificity. Moreover, there is no correlation between negative expression of p14^ARF and p16^INK4a proteins in

关 键 词：非小细胞肺癌 免疫组织化学 蛋白表达 P14^ARF P16^INK4A 

分 类 号：R734.2[医药卫生—肿瘤]